Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117829
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dc.contributor.authorPILLAI, V. V.eng
dc.contributor.authorSIQUEIRA, L. G. B.eng
dc.contributor.authorDAS, M.eng
dc.contributor.authorKEI, T. G.eng
dc.contributor.authorTU, L. N.eng
dc.contributor.authorHERREN, A. W.eng
dc.contributor.authorPHINNEY, B. S.eng
dc.contributor.authorCHEONG, S. H.eng
dc.contributor.authorHANSEN, P. J.eng
dc.contributor.authorSELVARAJ, V.eng
dc.date.accessioned2019-12-30T18:09:36Z-
dc.date.available2019-12-30T18:09:36Z-
dc.date.created2019-12-30
dc.date.issued2019
dc.identifier.citationBiology Open, v. 8, n. 5, article bio037937, 2019.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1117829-
dc.descriptionTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titlePhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-02-10T11:11:11Z
dc.subject.thesagroPlacentaeng
dc.subject.nalthesaurusTrophoblasteng
dc.subject.nalthesaurusBlastocysteng
dc.subject.nalthesaurusStem cellseng
dc.subject.nalthesaurusEmbryo implantationeng
dc.subject.nalthesaurusPregnancyeng
riaa.ainfo.id1117829eng
riaa.ainfo.lastupdate2020-02-10 -02:00:00
dc.identifier.doihttps://doi.org/10.1242/bio.037937eng
dc.contributor.institutionVIJU VIJAYAN PILLAI, Cornell University, Ithaca, NYeng
dc.contributor.institutionLUIZ GUSTAVO BRUNO SIQUEIRA, CNPGLeng
dc.contributor.institutionMOUBANI DAS, Cornell University, Ithaca, NYeng
dc.contributor.institutionTIFFANY G. KEI, Cornell University, Ithaca, NYeng
dc.contributor.institutionLAN N. TU, Cornell University, Ithaca, NYeng
dc.contributor.institutionANTHONY W. HERREN, University of Californiaeng
dc.contributor.institutionBRETT S. PHINNEY, University of Californiaeng
dc.contributor.institutionSOON HON CHEONG, Cornell University, Ithaca, NYeng
dc.contributor.institutionPETER J. HANSEN, University of Florida, Gainesville, FLeng
dc.contributor.institutionVIMAL SELVARAJ, Cornell University, Ithaca, NY.eng
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