Título:	Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts.
Autoria:	PILLAI, V. V. SIQUEIRA, L. G. B. DAS, M. KEI, T. G. TU, L. N. HERREN, A. W. PHINNEY, B. S. CHEONG, S. H. HANSEN, P. J. SELVARAJ, V.
Afiliação:	VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL MOUBANI DAS, Cornell University, Ithaca, NY TIFFANY G. KEI, Cornell University, Ithaca, NY LAN N. TU, Cornell University, Ithaca, NY ANTHONY W. HERREN, University of California BRETT S. PHINNEY, University of California SOON HON CHEONG, Cornell University, Ithaca, NY PETER J. HANSEN, University of Florida, Gainesville, FL VIMAL SELVARAJ, Cornell University, Ithaca, NY.
Ano de publicação:	2019
Referência:	Biology Open, v. 8, n. 5, article bio037937, 2019.
Conteúdo:	Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.
Thesagro:	Placenta
NAL Thesaurus:	Trophoblast Blastocyst Stem cells Embryo implantation Pregnancy
Digital Object Identifier:	https://doi.org/10.1242/bio.037937
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)