Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118145
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dc.contributor.authorMENDONÇA, J. F. M. DE
dc.contributor.authorVIEIRA, F. DE O.
dc.contributor.authorFONSECA, I.
dc.contributor.authorRIBEIRO, J. B.
dc.contributor.authorARCURI, E. F.
dc.contributor.authorBORGES, M. DE F.
dc.contributor.authorBORGES, C. A. V.
dc.contributor.authorSÁ, J. F. O. DE
dc.contributor.authorMARTINS, M. F.
dc.date.accessioned2024-02-06T12:33:36Z-
dc.date.available2024-02-06T12:33:36Z-
dc.date.created2020-01-05
dc.date.issued2019
dc.identifier.citationFood Science and Technology, Campinas, v. 39, p. 690-696, out. 2019.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1118145-
dc.descriptionReal-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella Typhimurium cells of 1 CFU/10 g of coalho cheese could be detected. The monoplex protocol enables the rapid and specific identification of viable Salmonella Typhimurium in coalho cheese, making it an alternative method for the quality and safety control of cheeses.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectLacticínios
dc.subjectViabilidade celular
dc.subjectAgentes intercalantes de DNA
dc.subjectDoenças transmitidas por alimentos
dc.subjectMicrorganismos patogênicos
dc.titleDetection of viable Salmonella Typhimurium and Staphylococcus aureus in coalho cheese by real-time PCR.
dc.typeArtigo de periódico
dc.subject.nalthesaurusCell viability
dc.subject.nalthesaurusDairy products
riaa.ainfo.id1118145
riaa.ainfo.lastupdate2024-02-06
dc.identifier.doihttps://doi.org/10.1590/fst.29318
dc.contributor.institutionJULIANA FRANÇA MONTEIRO DE MENDONÇA, Universidade Federal de Juiz de Fora; FELIPE DE OLIVEIRA VIEIRA, Universidade Federal de Juiz de Fora; ISABELA FONSECA, Instituto Federal Catarinense, Videira, SC; JOAO BATISTA RIBEIRO, CNPGL; EDNA FROEDER ARCURI, CNPGL; MARIA DE FATIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; JAQUELINE FLAVIANA OLIVEIRA DE SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; MARTA FONSECA MARTINS, CNPGL.
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