Title:	Detection of viable Salmonella Typhimurium and Staphylococcus aureus in coalho cheese by real-time PCR.
Authors:	MENDONÇA, J. F. M. DE VIEIRA, F. DE O. FONSECA, I. RIBEIRO, J. B. ARCURI, E. F. BORGES, M. DE F. BORGES, C. A. V. SÁ, J. F. O. DE MARTINS, M. F.
Affiliation:	JULIANA FRANÇA MONTEIRO DE MENDONÇA, Universidade Federal de Juiz de Fora; FELIPE DE OLIVEIRA VIEIRA, Universidade Federal de Juiz de Fora; ISABELA FONSECA, Instituto Federal Catarinense, Videira, SC; JOAO BATISTA RIBEIRO, CNPGL; EDNA FROEDER ARCURI, CNPGL; MARIA DE FATIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; JAQUELINE FLAVIANA OLIVEIRA DE SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; MARTA FONSECA MARTINS, CNPGL.
Date Issued:	2019
Citation:	Food Science and Technology, Campinas, v. 39, p. 690-696, out. 2019.
Description:	Real-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella Typhimurium cells of 1 CFU/10 g of coalho cheese could be detected. The monoplex protocol enables the rapid and specific identification of viable Salmonella Typhimurium in coalho cheese, making it an alternative method for the quality and safety control of cheeses.
NAL Thesaurus:	Cell viability Dairy products
Keywords:	Lacticínios Viabilidade celular Agentes intercalantes de DNA Doenças transmitidas por alimentos Microrganismos patogênicos
DOI:	https://doi.org/10.1590/fst.29318
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)