Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121822
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dc.contributor.authorPEREIRA, W. J.
dc.contributor.authorMELO, A. T. de O.
dc.contributor.authorCOELHO, A. S. G.
dc.contributor.authorRODRIGUES, F. A.
dc.contributor.authorMAMIDI, S.
dc.contributor.authorALENCAR, S. A. de
dc.contributor.authorLANNA, A. C.
dc.contributor.authorVALDISSER, P. A. M. R.
dc.contributor.authorBRONDANI, C.
dc.contributor.authorNASCIMENTO JUNIOR, I. R. do
dc.contributor.authorBORBA, T. C. de O.
dc.contributor.authorVIANELLO, R. P.
dc.date.accessioned2020-04-24T14:33:41Z-
dc.date.available2020-04-24T14:33:41Z-
dc.date.created2020-04-24
dc.date.issued2020
dc.identifier.citationGenetics and Molecular Biology, v. 43, n. 1, e20180259, 2020.
dc.identifier.issn1678-4685
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1121822-
dc.descriptionGenes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectFunctional genomics
dc.titleGenome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean.
dc.typeArtigo de periódico
dc.date.updated2020-04-24T14:33:41Z
dc.subject.thesagroFeijão
dc.subject.thesagroPhaseolus Vulgaris
dc.subject.thesagroDeficiência Hídrica
dc.subject.thesagroRaiz
dc.subject.thesagroFolha
dc.subject.nalthesaurusBeans
dc.subject.nalthesaurusSoil water deficit
dc.subject.nalthesaurusGenomics
dc.subject.nalthesaurusSingle nucleotide polymorphism
riaa.ainfo.id1121822
riaa.ainfo.lastupdate2020-04-24
dc.identifier.doihttps://doi.org/10.1590/1678-4685-gmb-2018-0259
dc.contributor.institutionWENDELL JACINTO PEREIRA, UFG; ARTHUR TAVARES DE OLIVEIRA MELO, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; FABIANA APARECIDA RODRIGUES; SUJAN MAMIDI, HUDSON ALPHA INSTITUTE FOR BIOTECHNOLOGY, Huntsville-AL; SERGIO AMORIM DE ALENCAR, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANNA CRISTINA LANNA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; CLAUDIO BRONDANI, CNPAF; IVANILDO RAMALHO DO NASCIMENTO JUNIOR, UNESP, Jaboticabal-SP; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
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