Título:	Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean.
Autoria:	PEREIRA, W. J. MELO, A. T. de O. COELHO, A. S. G. RODRIGUES, F. A. MAMIDI, S. ALENCAR, S. A. de LANNA, A. C. VALDISSER, P. A. M. R. BRONDANI, C. NASCIMENTO JUNIOR, I. R. do BORBA, T. C. de O. VIANELLO, R. P.
Afiliação:	WENDELL JACINTO PEREIRA, UFG; ARTHUR TAVARES DE OLIVEIRA MELO, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; FABIANA APARECIDA RODRIGUES; SUJAN MAMIDI, HUDSON ALPHA INSTITUTE FOR BIOTECHNOLOGY, Huntsville-AL; SERGIO AMORIM DE ALENCAR, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANNA CRISTINA LANNA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; CLAUDIO BRONDANI, CNPAF; IVANILDO RAMALHO DO NASCIMENTO JUNIOR, UNESP, Jaboticabal-SP; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Ano de publicação:	2020
Referência:	Genetics and Molecular Biology, v. 43, n. 1, e20180259, 2020.
Conteúdo:	Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
Thesagro:	Feijão Phaseolus Vulgaris Deficiência Hídrica Raiz Folha
NAL Thesaurus:	Beans Soil water deficit Genomics Single nucleotide polymorphism
Palavras-chave:	Functional genomics
ISSN:	1678-4685
Digital Object Identifier:	https://doi.org/10.1590/1678-4685-gmb-2018-0259
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAF)