Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123464
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dc.contributor.authorALVIM, B. F. M.
dc.contributor.authorSOUZA, A. V. de
dc.contributor.authorBRITO, A. L.
dc.contributor.authorFONSECA, P. T.
dc.contributor.authorSOARES, T. L
dc.contributor.authorSANTANA, J. R. F. de
dc.date.accessioned2020-06-26T11:11:18Z-
dc.date.available2020-06-26T11:11:18Z-
dc.date.created2020-06-25
dc.date.issued2020
dc.identifier.citationCiência Rural, v. 50, n. 7, e20190729, 2020.
dc.identifier.issn1678-4596
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1123464-
dc.descriptionSlow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectAmburana cearensis
dc.subjectPlanta nativa
dc.subjectUmburana-de- cheiro
dc.subjectCrescimento mínimo
dc.subjectAgente osmótico
dc.subjectConservação de germoplasma
dc.subjectRetardante de crescimento
dc.titleIn vitro conservation of Amburana cearensis (Fabaceae).
dc.typeArtigo de periódico
dc.subject.thesagroCaatinga
dc.subject.thesagroVegetação Nativa
dc.subject.thesagroPlanta Medicinal
dc.subject.thesagroCumaru
dc.subject.thesagroCultura de Tecido
dc.subject.thesagroRecurso Genético
dc.subject.nalthesaurusMedicinal plants
dc.subject.nalthesaurusVegetation
dc.subject.nalthesaurusTissue culture
riaa.ainfo.id1123464
riaa.ainfo.lastupdate2020-06-25
dc.identifier.doihttp://doi.org/10.1590/0103-8478cr20190729
dc.contributor.institutionBruno Freitas Matos Alvim; ANA VALERIA VIEIRA DE SOUZA, CPATSA; Alone Lima Brito; Priscila Tavares Fonseca; Taliane Leila Soares; José Raniere Ferreira de Santana.
Appears in Collections:Artigo em periódico indexado (CPATSA)

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