Title:	In vitro conservation of Amburana cearensis (Fabaceae).
Authors:	ALVIM, B. F. M. SOUZA, A. V. de BRITO, A. L. FONSECA, P. T. SOARES, T. L SANTANA, J. R. F. de
Affiliation:	Bruno Freitas Matos Alvim; ANA VALERIA VIEIRA DE SOUZA, CPATSA; Alone Lima Brito; Priscila Tavares Fonseca; Taliane Leila Soares; José Raniere Ferreira de Santana.
Date Issued:	2020
Citation:	Ciência Rural, v. 50, n. 7, e20190729, 2020.
Description:	Slow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process.
Thesagro:	Caatinga Vegetação Nativa Planta Medicinal Cumaru Cultura de Tecido Recurso Genético
NAL Thesaurus:	Medicinal plants Vegetation Tissue culture
Keywords:	Amburana cearensis Planta nativa Umburana-de- cheiro Crescimento mínimo Agente osmótico Conservação de germoplasma Retardante de crescimento
ISSN:	1678-4596
DOI:	http://doi.org/10.1590/0103-8478cr20190729
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)