Título:	Genetic characterization of a wheat association mapping panel relevant to brazilian breeding using a high-density single nucleotide polymorphism array.
Autoria:	Mellers, G. Aguilera, J. G. Bird, N. BONATO, A. L. V. BONOW, S. CAIERAO, E. CONSOLI, L. SANTANA, F. M. Simmonds, J. Steed, A. TORRES, G. A. M. Uauy, C. Wright, T. I. C. SCHEEREN, P. L. Nicholson, P. Cockram, J.
Afiliação:	Greg Mellers, *The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, United Kingdom Jorge González Aguilera, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom Nick Bird, The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, United Kingdom ANA LIDIA VARIANI BONATO, CNPT SANDRO BONOW, CPACT EDUARDO CAIERAO, CNPT LUCIANO CONSOLI, CNPT FLAVIO MARTINS SANTANA, CNPT James Simmonds, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom Andrew Steed, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom GISELE ABIGAIL MONTAN TORRES, CNPT Cristobal Uauy, ‡John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom Tally I. C. Wright, The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, United Kingdom,† PEDRO LUIZ SCHEEREN, CNPT Paul Nicholson, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom James Cockram, The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, United Kingdom.
Ano de publicação:	2020
Referência:	G3: Genes, Genomes, Genetics, v. 10, p. 2229-2239, July 2020
Conteúdo:	ABSTRACT Bread wheat (Triticum aestivum L.) is one of the world?s most important crops. Maintaining wheat yield gains across all of its major production areas is a key target toward underpinning global food security. Brazil is a major wheat producer in South America, generating grain yields of around 6.8 million tons per year. Here, we establish and genotype a wheat association mapping resource relevant to contemporary Brazilian wheat breeding programs. The panel of 558 wheat accessions was genotyped using an Illumina iSelect 90,000 single nucleotide polymorphism array. Following quality control, the final data matrix consisted of 470 accessions and 22,475 polymorphic genetic markers (minor allele frequency $5%, missing data ,5%). Principal component analysis identified distinct differences between materials bred predominantly for the northern Cerrado region, compared to those bred for southern Brazilian agricultural areas. We augmented the genotypic data with 26 functional Kompetitive Allele-Specific PCR (KASP) markers to identify the allelic combinations at genes with previously known effects on agronomically important traits in the panel. This highlighted breeding targets for immediate consideration ? notably, increased Fusarium head blight resistance via the Fhb1 locus. To demonstrate the panel?s likely future utility, genome-wide association scans for several phenotypic traits were undertaken. Significant (Bonferroni corrected P , 0.05) marker-trait associations were detected for Fusarium kernel damage (a proxy for type 2 Fusarium resistance), identifying previously known quantitative trait loci in the panel. This association mapping panel represents an important resource for Brazilian wheat breeding, allowing future genetic studies to analyze multiple agronomic traits within a single genetically diverse population.
NAL Thesaurus:	Wheat Breeding
Palavras-chave:	Genetic Characterization Brazilian Breeding Single Nucleotide Polymorphism Array Single nucleotide polymorphism (SNP) Genotyping quantitative trait locus (QTL) Genome-wide association scan (GWAS) Genetic diversity Polyploid crop Fusarium head blight (FHB)
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPT)