Título:	Genetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach.
Autoria:	AFONSO, J. FORTES, M. R. S. REVERTER, A. DINIZ, W. J. da S. CESAR, A. S. M. LIMA, A. O. de PETRINI, J. SOUZA, M. de S. COUTINHO, L. L. MOURÃO, G. B. ZERLOTINI NETO, A. GROMBONI, C. F. NOGUEIRA, A. R. A. REGITANO, L. C. de A.
Afiliação:	JULIANA AFONSO, UFSCar; MARINA RUFINO SALINAS FORTES, The University of Queensland; ANTONIO REVERTER, Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Brisbane; WELLISON JARLES DA SILVA DINIZ, UFSCar; ALINE SILVA MELLO CESAR, Esalq/USP; ANDRESSA OLIVEIRA DE LIMA, UFSCar; JULIANA PETRINI, UNIFAL; MARCELA M. DE SOUZA, Iowa State University; LUIZ LEHMANN COUTINHO, Esalq/USP; GERSON BARRETO MOURÃO, Esalq/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; CAIO FERNANDO GROMBONI, Bahia Federal Institute of Education, Science and Technology, Ilhéus; ANA RITA ARAÚJO NOGUEIRA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2020
Referência:	Scientific Reports, v. 10, n. 1, p. 1-16, 2020
Conteúdo:	Mineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF was adopted to determine the regulatory impact of genes and miRNAs expression over the GEBVs for the mineral amounts. We also investigated over-represented pathways, as well as pieces of evidences from previous studies carried in the same population and in the literature, to determine regulatory genes for the mineral amounts. For example, NOX1 expression level was positively correlated to Zinc and has been described as Zinc-regulated in humans. Based on our approach, we were able to identify genes, miRNAs and pathways not yet described as underlying mineral amount. The results support the hypothesis that extracellular matrix interactions are the core regulator of mineral amount in muscle cells. Putative regulators described here add information to this hypothesis, expanding the knowledge on molecular relationships between gene expression and minerals.
Thesagro:	Bos Indicus Gado Nelore
NAL Thesaurus:	Cattle Gene expression
Palavras-chave:	Expressão gênica
Digital Object Identifier:	https://doi.org/10.1038/s41598-020-65454-7
Notas:	Article: 8436.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPTIA)