Título:	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
Autoria:	CARRIERO, M. M. HENRIQUE SILVA, F. MEIRA, C. M. GATO, I. M. Q. CAETANO, A. R. LOBO, F. P. ALVES, A. L. VARELA, E. S. MAIA, A. A. M.
Afiliação:	MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.
Ano de publicação:	2020
Referência:	Genetics and Molecular Biology, v. 43, n. 4, e20200006, 2020.
Conteúdo:	In the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.
Thesagro:	Peixe Pacu Infecção
NAL Thesaurus:	Gene expression Piaractus mesopotamicus Fish Interleukins
Palavras-chave:	Bacterial infection Fish immunology South American fish
ISSN:	1678-4685
Digital Object Identifier:	https://doi.org/10.1590/1678-4685-gmb-2020-0006
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPASA)