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dc.contributor.authorGRUNENNVALDT, R. L.
dc.contributor.authorDEGENHARDT, J.
dc.contributor.authorBROOKS, P.
dc.contributor.authorTOMASI, J. de C.
dc.contributor.authorHANSEL, F. A.
dc.contributor.authorTRAN, T.
dc.contributor.authorGOMES, E. N.
dc.contributor.authorDESCHAMPS, C.
dc.date.accessioned2020-11-12T19:28:38Z-
dc.date.available2020-11-12T19:28:38Z-
dc.date.created2020-11-12
dc.date.issued2020
dc.identifier.citationAnais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126527-
dc.descriptionIlex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectBiomass accumulation
dc.subjectPlant growth regulators
dc.subjectErva mate
dc.subjectAtividade antioxidante
dc.subjectAcúmulo de biomassa
dc.titleCallus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification.
dc.typeArtigo de periódico
dc.subject.thesagroIlex Paraguariensis
dc.subject.thesagroSacarose
dc.subject.thesagroRegulador de Crescimento
dc.subject.nalthesaurusYerba mate
dc.subject.nalthesaurusSucrose
dc.subject.nalthesaurusAntioxidant activity
riaa.ainfo.id1126527
riaa.ainfo.lastupdate2020-11-23 -02:00:00
dc.identifier.doihttps://doi.org/10.1590/0001-3765202020181251
dc.contributor.institutionRENATA LÚCIA GRUNENNVALDT, UFPR
dc.contributor.institutionJULIANA DEGENHARDT, CNPFeng
dc.contributor.institutionPETER BROOKS, University of The Sunshine Coasteng
dc.contributor.institutionJÉSSICA DE CÁSSIA TOMASI, UFPReng
dc.contributor.institutionFABRICIO AUGUSTO HANSEL, CNPFeng
dc.contributor.institutionTRONG TRAN, University of The Sunshine Coasteng
dc.contributor.institutionERIK N. GOMES, The State University of New Jerseyeng
dc.contributor.institutionCÍCERO DESCHAMPS, UFPR.eng
Appears in Collections:Artigo em periódico indexado (CNPF)

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