Title:	Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation.
Authors:	GRUNENNVALDT, R. L. DEGENHARDT, J. BROOKS, P. TOMASI, J. de C. HANSEL, F. A. TRAN, T. GOMES, E. N. DESCHAMPS, C.
Affiliation:	RENATA LÚCIA GRUNENNVALDT, UFPR JULIANA DEGENHARDT, CNPF PETER BROOKS, University of The Sunshine Coast JÉSSICA DE CÁSSIA TOMASI, UFPR FABRICIO AUGUSTO HANSEL, CNPF TRONG TRAN, University of The Sunshine Coast ERIK N. GOMES, The State University of New Jersey CÍCERO DESCHAMPS, UFPR.
Date Issued:	2020
Citation:	Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p.
Description:	Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
Thesagro:	Ilex Paraguariensis Sacarose Regulador de Crescimento
NAL Thesaurus:	Yerba mate Sucrose Antioxidant activity
Keywords:	Biomass accumulation Plant growth regulators Erva mate Atividade antioxidante Acúmulo de biomassa
DOI:	https://doi.org/10.1590/0001-3765202020181251
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPF)