Título:	Efficiency of coffee seeds rna extraction protoco using rna integrity number analysis.
Autoria:	SANTOS, F. C. ROSA, S. D. V. F. da GROTEWOLD, E. PADILHA, L.
Afiliação:	The Ohio State University; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; The Ohio State University; LILIAN PADILHA, CNPCa.
Ano de publicação:	2014
Referência:	In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.
Conteúdo:	Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
Thesagro:	Extração RNA Semente Café
Tipo do material:	Resumo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Resumo em anais de congresso (SAPC)