Title:	Synthesis of chitosan polysaccharide nanoparticles for skin grape extract stabilization.
Authors:	SILVA, C. B. da DUARTE, D. S. SOUZA, A. V. de ASSIS, O. B. G. de BRITTO, D. de
Affiliation:	Cristina Barbosa da Silva; Dirliane Santos Duarte; ANA VALERIA VIEIRA DE SOUZA, CPATSA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; DOUGLAS DE BRITTO, CPATSA.
Date Issued:	2020
Citation:	Brazilian Journal of Development, v. 6, n. 4, p. 17913-17930, 2020.
Description:	Nanoparticles obtained by chitosan and tripolyphosphatehas been largely studied due to its entrapment characteristics. However, several parameters can influence the synthesis and encapsulationprocesses. In this way, this work proposed to study the nanoparticle formation with chitosan and tripolyphosphate in function of the acid concentration (1.0; 0.5 and0.1%) and the kind of the acid (hydrochloric and acetic). Conductometric and Potentiometric techniques showed that the reactional system was very dependent on the pH variation. The best condition, both for HCl and acetic acid, was achieved for systems with lower pH variation. For such systems, Dynamic Light Scattering technique showed formation of nanoparticle with the lowest size (135±7 nm) and higher stability (zeta potential near 30 mV). Also, by Scanning Electronic Microscopy, such systems exhibited good morphological aspect, with nanoparticles having spherical shape and lower level of agglomeration. The cryoprotection with sucrose solution before freeze-drying was fundamental to get samples with the improved re-suspension ability and preserved nanoparticle character. By UV-visible spectrophotometry, both HCl and acetic acid-based systems resulted in nanoparticles with good polyphenols encapsulation efficiency (~70%). The release profile was pH-dependent. In this way, the chitosan and tripolyphosphate ionic gelification need an accurate pH control both in the synthesis and in the encapsulation-release processes.
Thesagro:	Uva
NAL Thesaurus:	Grapes
Keywords:	Hidrogel Polieletrólito Reticulação iônica Polifenóis Encapsulamento Nanopartículas Casca de uva Quitosana
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)