Título:	Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation.
Autoria:	PETRÔNIO, M. S. BARROS-ALEXANDRINO, T. T. LIMA, A. M. F. ASSIS, O. B. G. de INOUE-NAGATA, A. K. NAKASU, E. Y. T. TIERA, M. J. PILON, L.
Afiliação:	ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ALICE KAZUKO INOUE NAGATA, CNPH; ERICH YUKIO TEMPEL NAKASU, CNPH; LUCIMEIRE PILON, CNPH.
Ano de publicação:	2022
Referência:	Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022.
Páginas:	5266 - 5279
Conteúdo:	Technologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection.
Palavras-chave:	Chitosan nanocarrier Tomato mosaic virus (ToMV) DsRNA phytotoxicity assay
Digital Object Identifier:	https://doi.org/10.33263/BRIAC124.52665279
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPDIA)