Title:	Production of Purpureocillium lilacinum and Pochonia chlamydosporia by submerged liquid fermentation and bioactivity against Tetranychus urticae and Heterodera glycines through seed inoculation.
Authors:	SILVA, D. M. SOUZA, V. H. M. de MORAL, R. de A. DELALIBERA JÚNIOR, I. MASCARIN, G. M.
Affiliation:	DANIELA MILANEZ SILVA, ESALQ-USP; VICTOR HUGO MOURA DE SOUZA, University of Cambridge; RAFAEL DE ANDRADE MORAL, Maynooth University; ITALO DELALIBERA JÚNIOR, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA.
Date Issued:	2022
Citation:	Journal of Fungi, v. 8, n. 5, article 511, 2022.
Description:	Abstract: Pochoniachlamydosporia and Purpureocilliumlilacinum are fungal bioagents used for the sustainable management of plant parasitic nematodes. However, their production through submerged liquid fermentation and their use in seed treatment have been underexplored. Therefore, our goal was to assess the effect of different liquid media on the growth of 40 isolates of P. lilacinum and two of P. chlamydosporia. The most promising isolates tested were assessed for plant growth promotion and the control of the two-spotted spider mite (Tetranychus urticae) and the soybean cyst nematode (Heterodera glycines). Most isolates produced > 108 blastospores mL−1 and some isolates produced more than 104 microsclerotia mL−1. Microsclerotia of selected isolates were used to inoculate common bean (Phaseolus vulgaris L.) seeds in greenhouse trials. All fungal isolates reduced the T. urticae fecundity in inoculated plants through seed treatment, while P. chlamydosporia ESALQ5406 and P. lilacinum ESALQ2593 decreased cyst nematode population. Purpureocillium lilacinum was more frequently detected in soil, whereas P. chlamydosporia colonized all plant parts. Pochonia chlamydosporia ESALQ5406 improved the root development of bean plants. These findings demonstrate the possibility of producing submerged propagules of P. chlamydosporia and P. lilacinum by liquid culture, and greenhouse trials support the applicability of fungal microsclerotia in seed treatment to control P. vulgaris pests.
Thesagro:	Controle Biológico Fungo Para Controle Biológico Fermentação Ácaro Tetranychus Urticae Nematóide Heterodera Glycines
NAL Thesaurus:	Biological control Biological control agents Liquid state fermentation Seed treatment Cyst nematodes Mites
Keywords:	Microsclerotia Soybean cyst nematode Two-spotted spider mite
ISSN:	2309-608X
DOI:	https://doi.org/10.3390/jof8050511
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)