Title:	Gene expression, biochemical and physiological activities in evaluating melon seed vigor through ethanol release.
Authors:	ORNELLAS, F. L. S. SOUSA, A. O. de PIROVANI, C. P. ARAÚJO, M. do N COSTA, D. S. da DANTAS, B. F. BARBOSA, R. M.
Affiliation:	FÁBIO LUIZ SANTOS ORNELLAS, Universidade Estadual de Santa Cruz; AURIZANGELA OLIVEIRA DE SOUSA, Universidade Federal do Oeste da Bahia; CARLOS PRIMINHO PIROVANI, Universidade Estadual de Santa Cruz; MARCELO DO NASCIMENTO ARAÚJO; DENIS SANTIAGO DA COSTA, Instituto Federal de Mato Grosso do Sul; BARBARA FRANCA DANTAS, CPATSA; RAFAEL MARANI BARBOSA, Universidade Estadual de Santa Cruz.
Date Issued:	2020
Citation:	Scientia Horticulturae, v. 261, 108884, 2020.
Description:	The aim of the present study was to establish parameters to evaluate melon seeds vigor by ethanol release test using a modified ethylometer and correlate with physiological seed quality, gene expression and enzyme activities alterations. To perform this trial, six seeds lots of yellow melon were submitted to determinations such as moisture content, germination assay and vigor tests. 25 and 50 seeds soaked with 0.5 mL of distilled water and tested after 1, 2, 3, 4, 5, 6 and 24 h at 40 °C. After 0, 6 and 24 h of imbibition, the high and low vigor lots were evaluated for the following enzyme activity: superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and alcoholic dehydrogenase (ADH). Accumulation of ADH protein was verified using Western Blotting hybridization technique with the primary Anti-ADH antibody. The release of ethanol in 25 seeds, soaked in 0.5 mL of distilled water for 6 h, proved efficient for vigor evaluation and can be related to other traditionally used tests. Enzyme activity varied according to the vigor of the seed lot. For the protein profile, there was variation in the accumulation of proteins of specific molecular weights throughout the soaking periods. There was also variation in expression according to accumulation of ADH protein. The biochemical and molecular tests corroborate with the establishment of the ethanol test as an additional tool to evaluate seed vigor.
Thesagro:	Semente Cucumis Melo Enzima Germinação Melão
NAL Thesaurus:	Enzymes Seed germination
Keywords:	Etilômetro Vigor de sementes de melão
DOI:	https://doi.org/10.1016/j.scienta.2019.108884
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)