Título:	A novel soybean hairy root system for gene functional validation.
Autoria:	PEREIRA, B. M. ARRAES, F. MARTINS, A. C. Q. ALVES, N. S. F. MELO, B. P. MORGANTE, C. V. SARAIVA, M. A. P. SA, M. F. G. de GUIMARAES, P. M. BRASILEIRO, A. C. M.
Afiliação:	BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ BRUNO PAES MELO, LONGPING HIGH TECH CAROLINA VIANNA MORGANTE, CPATSA MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA MARIA FATIMA GROSSI DE SA, CENARGEN PATRICIA MESSEMBERG GUIMARAES, CENARGEN ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN.
Ano de publicação:	2023
Referência:	Plos One, v. 18, n. 5, e0285504, 2023.
Conteúdo:	Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
Thesagro:	Glycine Max Soja Melhoramento Genético Vegetal Meloidogyne spp
NAL Thesaurus:	Soybeans
Palavras-chave:	Transgenic soybean Agrobacterium rhizogenes
Digital Object Identifier:	https://doi. org/10.1371/journal.pone.0285504
Notas:	Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)