Título:	Effect of Monocerin, a fungal secondary metabolite, on endothelial cells.
Autoria:	GOMES, T. C. CONRADO, R. OLIVEIRA, R. C. de SELARI, P. J. R. G. MELO, I. S. de ARAÚJO, W. L. MARIA, D. A. SOUZA, A. O. de
Afiliação:	TAINAH COLOMBO GOMES, INSTITUTO BUTANTAN; RAFAEL CONRADO, INSTITUTO BUTANTAN; RODRIGO CARDOSO DE OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; PRISCILA JANE ROMANO GONÇALVES SELARI, INSTITUTO FEDERAL GOIANO; ITAMAR SOARES DE MELO, CNPMA; WELINGTON LUIZ ARAÚJO, UNIVERSIDADE DE SÃO PAULO; DURVANEI AUGUSTO MARIA, INSTITUTO BUTANTAN; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN.
Ano de publicação:	2023
Referência:	Toxins v. 15, n. 5, article 344, 2023.
Conteúdo:	Abstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine.
Thesagro:	Cróton Caatinga Fungo
NAL Thesaurus:	Endophytes Fungi Exserohilum Medicinal fungi Secondary metabolites Cell viability Cell senescence
Palavras-chave:	Monocerin
ISSN:	2072-6651
Digital Object Identifier:	https://doi.org/10.3390/toxins15050344
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMA)