Title:	Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.
Authors:	MENDES, L. W. RAAIJMAKERS, J. M. HOLLANDER, M. de SEPO, E. EXPÓSITO, R. G. CHIORATO, A. F. MENDES, R. TSAI, S. M. CARRIÓN, V. J.
Affiliation:	LUCAS WILLIAM MENDES, UNIVERSIDADE DE SÃO PAULO; JOS M RAAIJMAKERS, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; EDIS SEPO; RUTH GÓMEZ EXPÓSITO, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW; ALISSON FERNANDO CHIORATO, INSTITUTO AGRONÔMICO DE CAMPINAS; RODRIGO MENDES, CNPMA; SIU MUI TSAI, UNIVERSIDADE DE SÃO PAULO; VICTOR J CARRIÓN, NETHERLANDS INSTITUTE OF ECOLOGY NIOO-KNAW.
Date Issued:	2023
Citation:	Environmental Microbiome, v. 18, n. 1, article 68, 2023.
Description:	Abstract: Background - Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a fox-susceptible and fox-resistant common bean cultivar. -- Results: Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion.
Thesagro:	Rizosfera Feijão Raiz Bactéria Controle Biológico
NAL Thesaurus:	Beans Rhizosphere bacteria Transcriptome
Keywords:	Plant-microbe interaction Endosphere Metagenome
ISSN:	2524-6372
DOI:	https://doi.org/10.1186/s40793-023-00524-7
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)