Título:	UPLC-MS-ESI-QTOF analysis and antifungal activity of aqueous extracts of Spondias tuberosa.
Autoria:	SANTOS, A. T. L. dos CARNEIRO, J. N. P. CRUZ, R. P. da SALES, D. L. PINHEIRO, J. C. A. FREITAS, M. A. de KERNTOPF, M. R. DELMONDES, G. de A. RIBEIRO, P. R. V. BRITO, E. S. de BATISTA, F. L. A. MAGALHÃES, F. E. A. PITA NETO, I. C. BRAGA, M. F. B. M. KOWALSKI, R. KOWALSKA, G. SZOPA, A. BAJ, T. COUTINHO, H. D. M.
Afiliação:	ANTONIA THASSYA LUCAS DOS SANTOS, UNIVERSIDADE REGIONAL DO CARIRI JOARA NÁLYDA PEREIRA CARNEIRO, UNIVERSIDADE REGIONAL DO CARIRI RAFAEL PEREIRA DA CRUZ, UNIVERSIDADE REGIONAL DO CARIRI DÉBORA LIMA SALES, UNIVERSIDADE REGIONAL DO CARIRI JACQUELINE COSMO ANDRADE-PINHEIRO, UNIVERSIDADE REGIONAL DO CARIRI MARIA AUDILENE DE FREITAS, UNIVERSIDADE FEDERAL DE PERNAMBUCO MARTA REGINA KERNTOPF, UNIVERSIDADE REGIONAL DO CARIRI GYLLYANDESON DE ARAÚJO DELMONDES, UNIVERSIDADE REGIONAL DO CARIRI PAULO RICELI VASCONCELOS RIBEIRO, CNPAT EDY SOUSA DE BRITO, CNAT FRANCISCO LUCAS ALVES BATISTA, UNIVERSIDADE REGIONAL DO CARIRI FRANCISCO ERNANI ALVES MAGALHÃES, UNIVERSIDADE ESTADUAL DO CEARÁ IVO CAVALCANTE PITA NETO, FACULDADE CECAPE MARIA FLAVIANA BEZERRA MORAIS-BRAGA, UNIVERSIDADE REGIONAL DO CARIRI RADOSŁAW KOWALSKI, UNIWERSYTET PRZYRODNICZNY W LUBLINIE GRAZYNA KOWALSKA, UNIWERSYTET PRZYRODNICZNY W LUBLINIE ALEKSANDRA SZOPA, UNIWERSYTET MEDYCZNY W LUBLINIE TOMASZ BAJ, UNIWERSYTET MEDYCZNY W LUBLINIE HENRIQUE DOUGLAS MELO COUTINHO, UNIVERSIDADE REGIONAL DO CARIRI.
Ano de publicação:	2023
Referência:	Molecules, Basel, v. 28, n. 1, art. 305, Jan. 2023.
Páginas:	14 p.
Conteúdo:	This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ?16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association.
Thesagro:	Umbu Spondias Tuberosa Cromatografia
NAL Thesaurus:	Flavonoids Fluconazole Ultra-performance liquid chromatography Mass spectrometry Electrospray ionization mass spectrometry
Palavras-chave:	Popular medicine
Digital Object Identifier:	https://doi.org/10.3390/molecules28010305
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAT)