Title:	Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.
Authors:	COSTA, L. S. A. S. FARIA, M. R. de CHIARAMONTE, J. B. MENDES, L. W. SEPO, E. HOLLANDER, M. de FERNANDES, J. M. C. CARRIÓN, V. J. BETTIOL, W. MAUCHLINE, T. H. RAAIJMAKERS, J. M. MENDES, R.
Affiliation:	LILIAN S. ABREU SOARES COSTA, NETHERLANDS INSTITUTE OF ECOLOGY; MÍRIAN RABELO DE FARIA; JOSIANE BARROS CHIARAMONTE; LUCAS W. MENDES, UNIVERSIDADE DE SÃO PAULO; EDIS SEPO, NETHERLANDS INSTITUTE OF ECOLOGY; MATTIAS DE HOLLANDER, NETHERLANDS INSTITUTE OF ECOLOGY; JOSE MAURICIO CUNHA FERNANDES, CNPT; VÍCTOR J. CARRIÓN, UNIVERSIDAD DE MÁLAGA; WAGNER BETTIOL, CNPMA; TIM H. MAUCHLINE, ROTHAMSTED RESEARCH; JOS M. RAAIJMAKERS, LEIDEN UNIVERSITY; RODRIGO MENDES, CNPMA.
Date Issued:	2023
Citation:	Environmental Microbiome, v. 18, n. 1, p., 2023.
Description:	Abstract: Background -Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen. Results: The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides. Conclusion: Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.
Thesagro:	Doença Fúngica Rizosfera Microbiologia do Solo Controle Microbiano
NAL Thesaurus:	Wheat Fungal diseases of plants Bipolaris sorokiniana Suppressive soils Microbiome Bacterial communities Fungal communities
Keywords:	Soilborne pathogens Plant disease suppression Soil microbiome
DOI:	https://doi.org/10.1186/s40793-023-00529-2
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPT)