Título:	A water-based ultrasound-assisted extraction system to obtain natural blue colorant from Genipap (Genipa americana L.).
Autoria:	BENVENUTTI, L. ROVARIS, B. C. OLIVEIRA, D. DE RIBEIRO, P. R. V. BRITO, E. S. de ZIELINSKI, A. A. F.
Afiliação:	LAÍS BENVENUTTI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; BEATRIZ CESA ROVARIS, UNIVERSIDADE FEDERAL DE SANTA CATARINA; DÉBORA DE OLIVEIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNAT; ACÁCIO ANTONIO FERREIRA ZIELINSKI, UNIVERSIDADE FEDERAL DE SANTA CATARINA.
Ano de publicação:	2024
Referência:	Food Chemistry Advances, v. 4, June, 100704, 2024.
Conteúdo:	This study aimed to optimize the extraction of a blue colorant using ultrasound-assisted technology employing water as the solvent from genipap, an Amazon fruit. Process conditions were optimized by multiresponse analysis through the antioxidant activity by the ferric reducing antioxidant potential (FRAP) assay, the total phenolic content, and the b* and C* color parameters. The optimized conditions of 70 °C, 325 W, solid: solvent ratio of 1:40 (m/v), and time of 10 min were validated externally at a level of 90 % confidence. The composition of aqueous blue extract obtained under the greatest conditions was evaluated by ultra-performance liquid chromatography (UPLC). Besides, in vitro bioactivities such as digestive enzymatic inhibitions assays, and antiproliferative activity through human cells assays were evaluated. Genipin, geniposide, and other ten iridoids were identified in the aqueous blue extract. The natural colorant obtained presented the capacity of inhibiting enzymes involved in lipid and glucose digestion, suggesting a potential control of obesity and glucose levels in blood without showing cytotoxicity. Therefore, this study presented, for the first time, the optimal operational conditions to obtain a natural blue colorant from genipap in a green approach with interesting biological properties for the food and pharmaceutical industries.
NAL Thesaurus:	Mass spectrometry Bioactive properties Cytotoxicity
Palavras-chave:	Natural pigment Blue colorant Green extraction Pigmento natural Citotoxicidade Corante azul Extração verde Espectrometria de massa Propriedades bioativas
Digital Object Identifier:	10.1016/j.focha.2024.100704
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAT)