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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1166549
Title: | A robust method for quantifying 42 phenolic compounds by RP-HPLC/DAD: columns performance and characterization of Brazilian Citrus peels. |
Authors: | LIMA, M. dos S.![]() ![]() MONTEIRO, L. I. da S. ![]() ![]() CARVALHO, A. J. de B. ![]() ![]() BASTOS, D. C. ![]() ![]() PIMENTEL, T. C. ![]() ![]() PIMENTEL, T. C. ![]() ![]() MAGNANI, M. ![]() ![]() |
Affiliation: | MARCOS DOS SANTOS LIMA, INSTITUTO FEDERAL DO SERTÃO PERNAMBUCANO LARISSA IRIS DA SILVA MONTEIRO, UNIVERSIDADE FEDERAL DA PARAÍBA ANA JÚLIA DE BRITO ARAÚJO CARVALHO, INSTITUTO FEDERAL DO SERTAO ̃ PERNAMBUCANO DEBORA COSTA BASTOS, CPATSA TATIANA COLOMBO PIMENTEL D, MARCIANE MAGNANI, INSTITUTO FEDERAL DO PARANÁ TATIANA COLOMBO PIMENTEL, INSTITUTO FEDERAL DO PARANÁ MARCIANE MAGNANI, UNIVERSIDADE FEDERAL DA PARAÍBA. |
Date Issued: | 2024 |
Citation: | Food Chemistry, v. 460, 140807, 2024. |
Description: | Reliable analytical methods are the basis for the elucidation of phenolic compounds in foods. This study aimed to optimize and validate a method for determining 42 phenolics using reverse-phase (RP) high-performance liquid chromatography (HPLC) coupled to diode-array-detector-DAD. The performance of two RP columns was eval- uated. The 150x4.6 mm 3-μm column showed superior separation quality, whereas 35 of the 42 phenolics showed a separation resolution ≥1.5. The method’s linearity, precision (coefficient variation< 3.09%), recovery (87.5-103.2%), specificity, limits of detection (0.04-0.25 mg/L), and quantification (0.06-0.25 mg/L) had acceptable ranges. Thirty phenolics were quantified in Citrus peels, mainly flavanones, flavanols, flavonols, and phenolic acids, highlighting the high values of hesperidin (535-35070 mg/kg) and naringin (26-36466 mg/kg). Lemon peels named ’Lisboa,’ ’Thaiti,’ ’Thaiti-2000’, and ’Thaiti-2001’ presented the main phenolics associated with antioxidant capacity. The presented method was robust for determining 42 phenolic compounds, offering a new approach for bioactive compound quantification in food matrices. |
Thesagro: | Fruta Cítrica |
NAL Thesaurus: | Antioxidant activity Liquid chromatography Bioactive compounds |
Keywords: | Casca de fruta Espécies cítricas Atividade antioxidante Cromatografia líquida Colunas de sílica Compostos bioativos |
Type of Material: | Artigo de periódico |
Access: | openAccess |
Appears in Collections: | Artigo em periódico indexado (CPATSA)![]() ![]() |
Files in This Item:
File | Description | Size | Format | |
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A-robust-method-for-quantifying-42-phenolic-compounds.pdf | 2.43 MB | Adobe PDF | ![]() View/Open |