Título:	A robust method for quantifying 42 phenolic compounds by RP-HPLC/DAD: columns performance and characterization of Brazilian Citrus peels.
Autoria:	LIMA, M. dos S. MONTEIRO, L. I. da S. CARVALHO, A. J. de B. BASTOS, D. C. PIMENTEL, T. C. PIMENTEL, T. C. MAGNANI, M.
Afiliação:	MARCOS DOS SANTOS LIMA, INSTITUTO FEDERAL DO SERTÃO PERNAMBUCANO LARISSA IRIS DA SILVA MONTEIRO, UNIVERSIDADE FEDERAL DA PARAÍBA ANA JÚLIA DE BRITO ARAÚJO CARVALHO, INSTITUTO FEDERAL DO SERTAO ̃ PERNAMBUCANO DEBORA COSTA BASTOS, CPATSA TATIANA COLOMBO PIMENTEL D, MARCIANE MAGNANI, INSTITUTO FEDERAL DO PARANÁ TATIANA COLOMBO PIMENTEL, INSTITUTO FEDERAL DO PARANÁ MARCIANE MAGNANI, UNIVERSIDADE FEDERAL DA PARAÍBA.
Ano de publicação:	2024
Referência:	Food Chemistry, v. 460, 140807, 2024.
Conteúdo:	Reliable analytical methods are the basis for the elucidation of phenolic compounds in foods. This study aimed to optimize and validate a method for determining 42 phenolics using reverse-phase (RP) high-performance liquid chromatography (HPLC) coupled to diode-array-detector-DAD. The performance of two RP columns was eval- uated. The 150x4.6 mm 3-μm column showed superior separation quality, whereas 35 of the 42 phenolics showed a separation resolution ≥1.5. The method’s linearity, precision (coefficient variation< 3.09%), recovery (87.5-103.2%), specificity, limits of detection (0.04-0.25 mg/L), and quantification (0.06-0.25 mg/L) had acceptable ranges. Thirty phenolics were quantified in Citrus peels, mainly flavanones, flavanols, flavonols, and phenolic acids, highlighting the high values of hesperidin (535-35070 mg/kg) and naringin (26-36466 mg/kg). Lemon peels named ’Lisboa,’ ’Thaiti,’ ’Thaiti-2000’, and ’Thaiti-2001’ presented the main phenolics associated with antioxidant capacity. The presented method was robust for determining 42 phenolic compounds, offering a new approach for bioactive compound quantification in food matrices.
Thesagro:	Fruta Cítrica
NAL Thesaurus:	Antioxidant activity Liquid chromatography Bioactive compounds
Palavras-chave:	Casca de fruta Espécies cítricas Atividade antioxidante Cromatografia líquida Colunas de sílica Compostos bioativos
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)