Título:	Establishment, multiplication, and biochemical analysis of embryogenic lines of the Amazonian palm Euterpe precatoria Mart. under suspension culture.
Autoria:	VASCONCELOS, A. D. M. FERREIRA, J. C. B. MEIRA, R. O. SILVA-CARDOSO, I. M. de A. NEVES, J. dos S. SOUZA, A. M. de SOUZA, A. L. X. de PEREIRA, J. E. S.
Afiliação:	ALEXANDRO DIAS MARTINS VASCONCELOS, UNIVERSITY OF BRASÍLIA; JÉSSICA CRISTINA BARBOSA FERREIRA, UNIVERSITY OF BRASÍLIA; RENNAN OLIVEIRA MEIRA; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; JOANE DOS SANTOS NEVES; ANDERSON MARCOS DE SOUZA, UNIVERSITY OF BRASÍLIA; ANDRE LUIS XAVIER DE SOUZA, CENARGEN; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN.
Ano de publicação:	2024
Referência:	Forests, v. 15, n. 10, 1804, 2024.
Conteúdo:	The palm Euterpe precatoria holds great social, cultural, and environmental importance. The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species using different explant sources. For this, eight lineages of E. precatoria embryogenic calluses were tested, with five in liquid medium Murashige and Skoog (MS) with 5 μM Picloram and three for comparison in semisolid medium MS with 20 μM Picloram and 5 μM 2iP. The growth curve was obtained by weighing the calli from 60 to 180 days of cultivation. The Gompertz model was applied, and growth kinetics were evaluated. At 100 days, the contents of total soluble sugars (TSSs) and total soluble proteins (TSPs) were determined. Principal components (PCA) were measured. According to the analysis of the data, the cultivation of E. precatoria lineages in liquid medium was successfully carried out, and the establishment was achieved. The model can be considered adequate since the R2 values found describe more than 90% of the growth kinetics of the lineages. In the liquid system, lineages L1 (from leaf explants and multiplied in semisolid medium—SM), L2 (from leaf explants and multiplied in SM), and L6 (from zygotic embryo explants and multiplied in liquid medium—LM) showed the shortest time to double the biomass accumulation. Multivariate analysis reveals a significant increase in masses in liquid cultures, represented by lineages L6 and L2. There was statistical difference in the amount of TSSs extracted; the highest TSS levels were observed in lineages cultivated in LM. The protein content found was very low, showing statistical differences among the lineages. In this work, the establishment and multiplication of embryogenic calli of E. precatoria are described for the first time, and they emerge as viable alternatives for the vegetative propagation of the species.
NAL Thesaurus:	Arecaceae Picloram Proteins
Palavras-chave:	Açaí-solteiro Euterpe spp Liquid medium Cell suspension Total soluble sugars
Digital Object Identifier:	https://doi.org/10.3390/f15101804
Notas:	Na publicação: Jonny Everson Scherwinski-Pereira.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CENARGEN)