Title:	Plasmid-tagged in transgenic beans identifies a haploid-specific gene in biobalistic process.
Authors:	SOARES, A. GROSSI DE SÁ, M. NEIVA, S. POTZEMBERG, M. RECH, E. FARIA, J. C. ROMANO, E. ARAGÃO, F. J. L.
Affiliation:	UNIVERSIDADE DE BRASÍLIA; UNIVERSIDADE CATÓLICA DE BRASÍLIA; UNIVERSIDADE CATÓLICA DE BRASÍLIA; UNIVERSIADE DE BRASÍLIA; ELIBIO LEOPOLDO RECH FILHO, CENARGEN; JOSIAS CORREA DE FARIA, CNPAF; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; FRANCISCO JOSE LIMA ARAGAO, CENARGEN.
Date Issued:	2003
Citation:	In: CONGRESSO BRASILEIRO DE GENETICA, 49., 2003, Águas de Lindóia. A dupla hélice do DNA: anais. Águas de Lindóia: Sociedade Brasileira de Genética, 2003.
Description:	Genes introduced in plants are normally transferred to the progeny. However, transformants where the transgenes fails to segregate in a Medelian fashion may occur. These altered patterns of segregation could be due to different mechanisms, including insertional mutagenesis of genes that are essential to gametophyte development. Considering the difficulties encountered in isolation of gamethophytes, insertional mutagenesis becomes an important alternative for isolation of haploid-expressed genes. The rati onale of this approach is that mutations that affect gametophyte viability and function lead to a defect in the transmission of gametes. Particularly megagametophytic mutated genes reduce the number of seeds per pod. Pollens of transgenic bean plants were analyzed by Alexander and histochemical GUS assay, in order to determine their viability. Phenotypic analysis of female gametophyte mutants is facilitated by the fact that this kind of mutation results in transgenic plants with pods presenting a reduced number of seeds.
Keywords:	Feijão transgênico Biobalistic process Plasmid Haploid-specific
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPAF)