Título:	Use of a one-step freezing protocol for boar sperm with distinct cryoprotectants.
Autoria:	PHILIPPE, M. G. QUIRINO, M. SCHUCH, M. SCHULTZ, C. VIEIRA, A. D. MONDADORI, R. G. THOMAZ JÚNIOR, L. MOREIRA, F. PERIPOLLI, V. MARQUES, M. G. BIANCHI, I.
Afiliação:	MAIKO GIORGI PHILIPPE, INSTITUTO FEDERAL CATARINENSE; MONIKE QUIRINO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; MARIAH SCHUCH, UNIVERSIDADE FEDERAL DE PELOTAS; CAROLINI SCHULTZ, INSTITUTO FEDERAL CATARINENSE; ARNALDO DINIZ VIEIRA, UNIVERSIDADE FEDERAL DE PELOTAS; RAFAEL GIANELLA MONDADORI, UNIVERSIDADE FEDERAL DE PELOTAS; THOMAZ LUCIA JÚNIOR, UNIVERSIDADE FEDERAL DE PELOTAS; FABIANA MOREIRA, INSTITUTO FEDERAL CATARINENSE; VANESSA PERIPOLLI, INSTITUTO FEDERAL CATARINENSE; MARIANA GROKE MARQUES, CNPSA; IVAN BIANCHI, INSTITUTO FEDERAL CATARINENSE.
Ano de publicação:	2024
Referência:	Ciência Rural, Santa Maria, v. 54, n. 1, ed. e20220090, 2024.
Conteúdo:	Abstract: The present study evaluated the cryoprotectant efficacy of dimethylacetamide (DMA) and ethylene glycol in a one-step protocol to freeze boar sperm. The sperm-rich portion of the ejaculates from two boars were collected once a week, for 10 weeks. After collection, the ejaculates were diluted (1:1; v/v) in the cooling extender. After determining their spermatozoa concentration, the ejaculates were pooled with the same number of spermatozoa from each boar and stabilized at 20 °C for 120 min. Distinct cryoprotectants were added to the cooling extender at 20 °C, at different concentrations, composing six treatments: 1.25% and 2.5% glycerol (control); 1.25% and 2.5% ethylene glycol; 2.5% and 5.0% DMA. The samples were stored in 0.25 mL straws, containing 35 × 106 spermatozoa. After 90 min at 20 °C, the straws were submitted to a cooling curve until 5 °C (0.3 to 0.5 °C/min) and kept at 5°C for 60 min. Freezing was conducted by placing the straws horizontally 5 cm above the liquid nitrogen for 10 min, followed by immersion on liquid nitrogen. After thawing at 37 °C for 30 seconds, sperm quality was evaluated through a computer-assisted semen analysis system and flow cytometry. Sperm motility was greater (P < 0.05) in treatments with 5.0% and 2.5% DMA (22.2 ± 2.6% and 20.0 ± 2.8%, respectively) than in treatment with 2.5% ethylene glycol (8.2 ± 1.0%). The integrity of the plasma membrane (P = 0.08) and mitochondrial membrane potential (P = 0.27) was similar among the treatments. The treatment with 2.5% ethylene glycol was the least efficient to maintain intact acrosome membrane (P < 0.01). Some kinetics parameters (DAP, DCL, DSL, VAP, VCL, VSL e ALH) were positively affected by 5.0% DMA. The one-step freezing protocol resulted in unsatisfactory boar sperm motility after thawing, regardless of the cryoprotectant.
NAL Thesaurus:	Swine Glycerol Ethylene glycol Cryopreservation
Palavras-chave:	Dimethylacetamide
Digital Object Identifier:	http://doi.org/10.1590/0103-8478cr20220090
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPSA)