Título:	Polymorphism in the C-mos gene and productive traits in captive-bred scorpion mud turtles.
Autoria:	FERREIRA, P. F. G. SILVA, R. A. HAMOY, I. G. MARQUES, L. C. SILVA, C. S. MARQUES, J. R. F. PEREIRA, W. L. A. SILVA FILHO, E.
Afiliação:	P. F. G. FERREIRA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; R. A. SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; I. G. HAMOY, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; L. C. MARQUES, UNIVERSIDADE DA AMAZÔNIA; C. S. SILVA, UNIVERSIDADE FEDERAL DO PARÁ; JOSE RIBAMAR FELIPE MARQUES, CPATU; W. L. A. PEREIRA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; E. SILVA FILHO, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA.
Ano de publicação:	2024
Referência:	Genetics and Molecular Research, v. 23, n. 3, gmr2377, 2024.
Conteúdo:	Kinosternon scorpioides is a freshwater turtle in need of conservation due to its vulnerability and population decline caused by predatory extraction from the natural environment and habitat destruction and degradation. Consequently, previous studies have focused on indicating the species’ productive potential through productive features associations that aid the captive breeding system, but no one has explored candidate genes for these traits through molecular markers. Therefore, the C-mos gene was evaluated by the polymerase chain reaction technique (PCR) to look for polymorphisms useful in genetic analyses of a population raised in the Embrapa Eastern Amazon captivity, given its role in the maturation and regulation of oocyte transition and skeletal muscle development. The aim was to look for these polymorphisms, characterize them, and link them with body weight (BW) and the average number of eggs (NE) traits. Thus, the DNA of 75 animals was extracted, sequenced, and the allelic and genotype frequency, observed and expected heterozygosity, inbreeding coefficients (FIS), and Hardy-Weinberg probability measures were assessed with GENEPOP software. The analysis of variance (ANOVA) one-way test at a 5% level of significance was used to check if BW and NE traits differ between different genotypes. The results revealed a non-synonymous polymorphism resulting in three genotypes: CC (49%), CT (42%), and TT (9%). However, these genotypes were not statistically associated with the body weight or number of eggs of the animals. Although this polymorphism is suitable to be used as a molecular marker in population genetic analysis involving species raised in captivity.
Thesagro:	Polimorfismo Criação em Cativeiro Gene
Palavras-chave:	Kinosternon scorpioides
Digital Object Identifier:	http://dx.doi.org/10.4238/gmr2377
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATU)