Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1175097
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorDANTAS, A. F.
dc.contributor.authorNUNES, T. C.
dc.contributor.authorGRYNBERG, P.
dc.contributor.authorBRASILEIRO, A. C. M.
dc.contributor.authorSALOMAO, A. N.
dc.contributor.authorJOSE, S. C. B. R.
dc.contributor.authorVIANELLO, R. P.
dc.contributor.authorPADUA, J. G.
dc.contributor.authorPAPPAS, M. de C. R.
dc.contributor.authorGIMENES, M. A.
dc.date.accessioned2025-04-24T17:47:40Z-
dc.date.available2025-04-24T17:47:40Z-
dc.date.created2025-04-24
dc.date.issued2025
dc.identifier.citationScientific Reports, v.15, 12407, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1175097-
dc.descriptionStored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectAged seeds
dc.subjectPhaseolus vulgaris L
dc.subjectRNA integrity number
dc.titleLong-term preserved bean seeds exhibit high RNA integrity and high germination potential.
dc.typeArtigo de periódico
riaa.ainfo.id1175097
riaa.ainfo.lastupdate2025-04-24
dc.identifier.doihttps://doi.org/10.1038/s41598-024-85030-7
dc.contributor.institutionALISSON FERREIRA DANTAS, NATIONAL COUNCIL FOR SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT; TAYARA COLINS NUNES, FEDERAL DISTRICT RESEARCH SUPPORT FOUNDATION; PRISCILA GRYNBERG, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; ANTONIETA NASSIF SALOMAO, CENARGEN; SOLANGE CARVALHO B ROVERI JOSE, CENARGEN; ROSANA PEREIRA VIANELLO, CNPAF; JULIANO GOMES PADUA, CENARGEN; MARILIA DE CASTRO RODRIGUES PAPPAS, CENARGEN; MARCOS APARECIDO GIMENES, CENARGEN.
Aparece en las colecciones:Artigo em periódico indexado (CENARGEN)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
s41598-024-85030-7.pdf1.74 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace