Título:	Long-term preserved bean seeds exhibit high RNA integrity and high germination potential.
Autor:	DANTAS, A. F. NUNES, T. C. GRYNBERG, P. BRASILEIRO, A. C. M. SALOMAO, A. N. JOSE, S. C. B. R. VIANELLO, R. P. PADUA, J. G. PAPPAS, M. de C. R. GIMENES, M. A.
Afiliación:	ALISSON FERREIRA DANTAS, NATIONAL COUNCIL FOR SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT; TAYARA COLINS NUNES, FEDERAL DISTRICT RESEARCH SUPPORT FOUNDATION; PRISCILA GRYNBERG, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; ANTONIETA NASSIF SALOMAO, CENARGEN; SOLANGE CARVALHO B ROVERI JOSE, CENARGEN; ROSANA PEREIRA VIANELLO, CNPAF; JULIANO GOMES PADUA, CENARGEN; MARILIA DE CASTRO RODRIGUES PAPPAS, CENARGEN; MARCOS APARECIDO GIMENES, CENARGEN.
Año:	2025
Referencia:	Scientific Reports, v.15, 12407, 2025.
Descripción:	Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests.
Palabras clave:	Aged seeds Phaseolus vulgaris L RNA integrity number
DOI:	https://doi.org/10.1038/s41598-024-85030-7
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CENARGEN)