Título:	Discovery and genome characterization of three New rhabdoviruses infecting Passiflora spp. in Brazil
Autor:	VIDAL, A. H. ABREU, A. C. R. DANTAS-FILHO, J. F. S. VIANNA, M. J. X. LACORTE, C. C. ABREU, E. F. M. FELIX, G. P. ALVES-FREITAS, D. M. T. PINHEIRO-LIMA, B. NOGUEIRA, I. FALEIRO, F. G. ROSA, R. C. C. JESUS, O. N. de SANCHES, M. M. SANTOS, Y. S. BLAWID, R. JIMÉNEZ, J. L. S. VASLIN, M. F. S. KITAJIMA, E. W. CAMPOS, M. de A. FONTENELE, R. S. VARSANI, A. MELO, F. L. RIBEIRO, S. da G.
Afiliación:	ANDREZA HENRIQUE VIDAL, UNIVERSIDADE DE BRASÍLIA; ANA CLARA RODRIGUES ABREU, UNIVERSIDADE DE BRASÍLIA; JORGE FLÁVIO SOUSA DANTAS-FILHO, UNIVERSIDADE DE BRASÍLIA; MONIQUE JACOB XAVIER VIANNA, UNIVERSIDADE DE BRASÍLIA; CRISTIANO CASTRO LACORTE, CENARGEN; EMANUEL FELIPE MEDEIROS ABREU, CENARGEN; GUSTAVO PEREIRA FELIX, UNIVERSIDADE DE BRASÍLIA; DIONE MENDES TEIXEIRA ALVES-FREITAS; BRUNA PINHEIRO-LIMA, BRUNA PINHEIRO-LIMA; ISADORA NOGUEIRA, UNIVERSIDADE DE BRASÍLIA; FABIO GELAPE FALEIRO, CPAC; RAUL CASTRO CARRIELLO ROSA, CNPAB; ONILDO NUNES DE JESUS, CNPMF; MARCIO MARTINELLO SANCHES, CNPGC; YAM SOUSA SANTOS, UNIVERSIDADE FEDERAL DE VIÇOSA (UFV); ROSANA BLAWID, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; JOSÉ LEONARDO SANTOS JIMÉNEZ, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; MAITE FREITAS SILVA VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; ELLIOT WATANABE KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MAGNOLIA DE ARAUJO CAMPOS, UNIVERSIDADE FEDERAL DE CAMPINA GRANDE; RAFAELA SALGADO FONTENELE, ARIZONA STATE UNIVERSITY; ARVIND VARSANI, ARIZONA STATE UNIVERSITY; FERNANDO LUCAS MELO, ONSITE GENOMICS; SIMONE DA GRACA RIBEIRO, CENARGEN.
Año:	2025
Referencia:	Viruses, v. 17, n. 5, 725, 2025.
Descripción:	This study aimed to explore the RNA viruses affecting Passiflora species in Brazil. Our results enhance the understanding of the viruses that infect Passiflora plants by identifying and characterizing three previously unrecognized viruses: Passiflora cytorhabdovirus (PFCV), Passiflora nucleorhabdovirus 1 (PaNV1), and Passiflora nucleorhabdovirus 2 (PaNV2). These rhabdoviruses were identified through high-throughput sequencing and validated by reverse transcription-polymerase chain reaction (RT-PCR) in various Passiflora species. PFCV has a genome organization 3′-N-P-P3-P4-M-G-P7-L-5′ and was classified as a novel member of the Gammacytorhabdovirus genus. A particularly noteworthy feature of PFCV is its glycoprotein, as the genomes of other gammarhabdoviruses do not contain this gene. PFCV has a high incidence across multiple locations and was identified in plants from Northeastern, Central, and Southeastern Brazil. PaNV1 with genome structure 3′-N-P-P3-M-G-L-5′ and PaNV2 with genome organization 3′-N-X-P-Y-M-G-L-5′ are new members of the Alphanucleorhabdovirus genus and have a more restricted occurrence. Importantly, all three viruses were found in mixed infections alongside at least one other virus. In situ observations confirmed mixed infections, with PaNV2 particles co-located in tissues with a potyvirus and a carlavirus. Phylogenetic and glycoprotein sequence similarity network analysis provided insights into their evolutionary placement and potential vector associations. These findings expand the known diversity of rhabdoviruses in Passiflora and contribute to the understanding of their evolution and epidemiology.
NAL Thesaurus:	Microscopy
Palabras clave:	Plant rhabdovirus High-throughput sequencing Virus particles Gammacytorhabdovirus Alphanucleorhabdovirus
DOI:	https://doi.org/10.3390/v17050725
Notas:	Na publicação: Cristiano Lacorte; Onildo Nunes Jesus; Simone Graça Ribeiro.
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CENARGEN)