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dc.contributor.authorCASTELO, R. M.eng
dc.contributor.authorOLIVEIRA, M. DE A.eng
dc.contributor.authorFURTADO, R. F.eng
dc.contributor.authorOLIVEIRA, B. P. DEeng
dc.contributor.authorMARTONI, L. V. L.eng
dc.contributor.authorMACHADO, T. F.eng
dc.contributor.authorMUNIZ, C. R.eng
dc.contributor.authorABREU, F. O. M. DA S.eng
dc.contributor.authorMACHADO, S. A. S.eng
dc.contributor.authorMELO, A. M. A.eng
dc.contributor.authorCHENG, H. N.eng
dc.contributor.authorBISWAS, A.eng
dc.contributor.authorALVES, C. R.eng
dc.date.accessioned2025-06-11T19:47:52Z-
dc.date.available2025-06-11T19:47:52Z-
dc.date.created2025-06-11
dc.date.issued2025
dc.identifier.citationBrazilian Journal of Microbiology, Jan. 2025
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1176555-
dc.descriptionCarbon dots in biosensing have advanced significantly, adding improvements to different detection techniques. In this study, an amperometric immunosensor for Salmonella Thyphimurium was designed using antibodies labeled with car- bon dots (Cdots) from pequi almond (Caryocar brasiliensis). Cdots were synthesized by pyrolysis and characterized by FTIR, UV/fluorescence, electrochemistry, zeta potential, and transmission electron microscopy (TEM). A particle size of 6.80 ± 2.13 nm was estimated, and the zeta potential was − 47.4 mV, indicating the preponderant presence of acidic groups, as confirmed by FTIR. The impedance evaluation of the response of biosensors assembled for live (Rct = 13.4 kΩ) and dead (Rct = 499.7 Ω) Salmonella showed a significant difference in their values, in agreement with chronoamperometric analyses, which had their current values drastically reduced from − 2.2 mA (live) to 0 mA (dead). An analytical curve for Salmonella was established with the limit of detection lower than 1 CFU/mL. This electrochemical biosensor using pequi carbon dots for antibody labeling showed promising results for detecting the pathogen. Thus, carbon dots can be used as substitutes for enzymes in labeling antibodies used in the design and production of sensors.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectCarbon dots
dc.subjectCaryocar brasiliensis Cambess
dc.subjectPequi nut
dc.subjectSalmonella Thyphimurium
dc.subjectPontos de carbono
dc.subjectImunosensor
dc.subjectNoz de pequi
dc.titleCarbon-dot pequi-nut in the development of immunosensor to detect pathogenic bacteria.
dc.typeArtigo de periódico
dc.subject.nalthesaurusImmunosensors
riaa.ainfo.id1176555
riaa.ainfo.lastupdate2025-06-11
dc.identifier.doihttps://doi.org/10.1007/s42770-025-01612-1
dc.contributor.institutionRACHEL MENEZES CASTELO, UNIVERSIDADE ESTADUAL DO CEARÁeng
dc.contributor.institutionMARÍLIA DE ALBUQUERQUE OLIVEIRA, RENORBIOeng
dc.contributor.institutionROSELAYNE FERRO FURTADO, CNPATeng
dc.contributor.institutionBRUNO PEIXOTO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO CARIRIeng
dc.contributor.institutionLUCAS VINICIUS LEITE MARTONI, UNIVERSIDADE DE SÃO PAULOeng
dc.contributor.institutionTEREZINHA FEITOSA MACHADO, CNPATeng
dc.contributor.institutionCELLI RODRIGUES MUNIZ, CNPATeng
dc.contributor.institutionFLÁVIA OLIVEIRA MONTEIRO DA SILVA ABREU, UNIVERSIDADE ESTADUAL DO CEARÁeng
dc.contributor.institutionSÉRGIO ANTÔNIO SPINOLA MACHADO, UNIVERSIDADE DE SÃO PAULOeng
dc.contributor.institutionAIRIS MARIA ARAÚJO MELO, INSTITUTO FEDERAL DO PIAUÍ CAMPUS PICOSeng
dc.contributor.institutionHUAI N. CHENG, SOUTHERN REGIONAL RESEARCH CENTEReng
dc.contributor.institutionATANU BISWAS, USDA AGRICULTURAL RESEARCH SERVICEeng
dc.contributor.institutionCARLUCIO ROBERTO ALVES, UNIVERSIDADE ESTADUAL DO CEARÁ.eng
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