Título:	Carbon-dot pequi-nut in the development of immunosensor to detect pathogenic bacteria.
Autoria:	CASTELO, R. M. OLIVEIRA, M. DE A. FURTADO, R. F. OLIVEIRA, B. P. DE MARTONI, L. V. L. MACHADO, T. F. MUNIZ, C. R. ABREU, F. O. M. DA S. MACHADO, S. A. S. MELO, A. M. A. CHENG, H. N. BISWAS, A. ALVES, C. R.
Afiliação:	RACHEL MENEZES CASTELO, UNIVERSIDADE ESTADUAL DO CEARÁ MARÍLIA DE ALBUQUERQUE OLIVEIRA, RENORBIO ROSELAYNE FERRO FURTADO, CNPAT BRUNO PEIXOTO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO CARIRI LUCAS VINICIUS LEITE MARTONI, UNIVERSIDADE DE SÃO PAULO TEREZINHA FEITOSA MACHADO, CNPAT CELLI RODRIGUES MUNIZ, CNPAT FLÁVIA OLIVEIRA MONTEIRO DA SILVA ABREU, UNIVERSIDADE ESTADUAL DO CEARÁ SÉRGIO ANTÔNIO SPINOLA MACHADO, UNIVERSIDADE DE SÃO PAULO AIRIS MARIA ARAÚJO MELO, INSTITUTO FEDERAL DO PIAUÍ CAMPUS PICOS HUAI N. CHENG, SOUTHERN REGIONAL RESEARCH CENTER ATANU BISWAS, USDA AGRICULTURAL RESEARCH SERVICE CARLUCIO ROBERTO ALVES, UNIVERSIDADE ESTADUAL DO CEARÁ.
Ano de publicação:	2025
Referência:	Brazilian Journal of Microbiology, Jan. 2025
Conteúdo:	Carbon dots in biosensing have advanced significantly, adding improvements to different detection techniques. In this study, an amperometric immunosensor for Salmonella Thyphimurium was designed using antibodies labeled with car- bon dots (Cdots) from pequi almond (Caryocar brasiliensis). Cdots were synthesized by pyrolysis and characterized by FTIR, UV/fluorescence, electrochemistry, zeta potential, and transmission electron microscopy (TEM). A particle size of 6.80 ± 2.13 nm was estimated, and the zeta potential was − 47.4 mV, indicating the preponderant presence of acidic groups, as confirmed by FTIR. The impedance evaluation of the response of biosensors assembled for live (Rct = 13.4 kΩ) and dead (Rct = 499.7 Ω) Salmonella showed a significant difference in their values, in agreement with chronoamperometric analyses, which had their current values drastically reduced from − 2.2 mA (live) to 0 mA (dead). An analytical curve for Salmonella was established with the limit of detection lower than 1 CFU/mL. This electrochemical biosensor using pequi carbon dots for antibody labeling showed promising results for detecting the pathogen. Thus, carbon dots can be used as substitutes for enzymes in labeling antibodies used in the design and production of sensors.
NAL Thesaurus:	Immunosensors
Palavras-chave:	Carbon dots Caryocar brasiliensis Cambess Pequi nut Salmonella Thyphimurium Pontos de carbono Imunosensor Noz de pequi
Digital Object Identifier:	https://doi.org/10.1007/s42770-025-01612-1
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAT)