Título:	Genetic diversity among acerola accessions collected in different brazilian states using ISSR markers.
Autoria:	NASCIMENTO, T. L. do SOUZA, S. S. SANTOS, M. de S. FELINTO FILHO, E. F. SILVA, R. de S. MELO, N. F. de SOUZA, F. de F.
Afiliação:	TIAGO L. DO NASCIMENTO; SIMONE S. SOUZA; MIKAELE DE S. SANTOS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; EZILDO F. FELINTO FILHO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; RAQUEL DE S. SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTA; NATONIEL FRANKLIN DE MELO, CPATSA; FLAVIO DE FRANCA SOUZA, CPATSA.
Ano de publicação:	2025
Referência:	Revista Caatinga, v. 38, e12697, 2025.
Conteúdo:	Commercial acerola (Malpighia emarginata Sessé & Moc. ex DC.) orchards in Brazil are typically established using only one or two genotypes, making them highly susceptible to biotic and abiotic stresses. Molecular characterization of available germplasm can help identify valuable alleles for the development of new cultivars. This study aimed to assess the genetic diversity of a representative sample of acerola germplasm cultivated in Brazil using inter-simple sequence repeat (ISSR) primers. Genomic DNA was extracted from 96 accessions conserved in Petrolina, Pernambuco state (PE) and amplified using 15 ISSR primers. The presence/absence of bands was scored to estimate allelic similarity based on the Jaccard index, and a dendrogram was constructed using the UPGMA method. Genetic variation within and between groups was quantified via analysis of molecular variance (AMOVA), using Genes, GenAlex and Cervus software. The accessions were grouped into 24 clusters, with ACO01 and MAR12 being the most divergent and Costa Rica and Flor Branca the most similar. The cluster analysis revealed substantial variation among individuals from the same region (81%), a result confirmed by AMOVA and the Shannon -Wiener diversity index. The ISSR primers effectively captured the genetic variation among the accessions studied. Given that the samples analyzed represent acerola germplasm cultivated across Brazil, these results provide valuable insights for the management of genetic resources and the development of acerola breeding programs
Thesagro:	Melhoramento Genético Vegetal Marcador Genético Marcador Molecular Acerola
NAL Thesaurus:	Malpighia emarginata Tree breeding Molecular genetics Genetic markers
Palavras-chave:	Melhoramento genético Divergência genética Marcadores ISSR
ISSN:	1983-2125 (online)
Digital Object Identifier:	http://dx.doi.org/10.1590/1983-21252025v3812697rc
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)