Título:	Progress and opportunities of In planta and topical RNAi for the biotechnological control of agricultural pests.
Autoria:	BASSO, M. F. VÁSQUEZ, D. D. N. PINTO, E. R. de C. PINHEIRO, D. H. CRUZ, B. MAKTURA, G. C. GUIDELLI, G. V. MARQUES-SOUZA, H. SA, M. F. G. de
Afiliação:	MARCOS FERNANDO BASSO, UNIVERSITY OF FLORENCE; DANIEL DAVID NORIEGA VÁSQUEZ, INCT PLANTSTRESS BIOTECH; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; DANIELE HELOÍSA PINHEIRO, INCT PLANTSTRESS BIOTECH; BREAD CRUZ, UNIVERSITY OF CAMPINAS (UNICAMP); GRAZIELLE CELESTE MAKTURA, UNIVERSITY OF CAMPINAS (UNICAMP); GIOVANNA VIEIRA GUIDELLI, UNIVERSITY OF CAMPINAS (UNICAMP); HENRIQUE MARQUES-SOUZA, UNIVERSITY OF CAMPINAS (UNICAMP); MARIA FATIMA GROSSI DE SA, CENARGEN.
Ano de publicação:	2025
Referência:	Agronomy, v. 15, n. 4, 859, 2025.
Conteúdo:	In planta RNAi or host-induced gene silencing (HIGS) has undergone significant advancements that have rendered it efficient and stable at the transgenerational level in plants for regulating host genes and targeting genes of insect pests and plant pathogens. Similarly, topical RNAi or spray-induced gene silencing (SIGS) has garnered considerable attention as an environmentally sustainable, selective, and alternative approach to chemical control of insect pests and plant pathogens. Several biotechnology companies and startups have focused their efforts on RNAi-based solutions for topical application in agriculture. Nevertheless, further technological advancements are required to enhance the efficacy of topical RNAi in agriculture, including improved dsRNA delivery systems, better target gene selection, and addressing biosafety regulatory issues. Herein, this review discusses key advances and bottlenecks in RNAi, and summarizes successful applications of these RNAi-based technologies in agriculture focusing on in planta and topical RNAi to control insect pests and plant pathogens. Furthermore, this review delves into the patenting landscape, biosafety considerations, risk evaluations, and the current regulatory status of RNAi in Latin America. Finally, it explores the contributions of RNAi to plant science, food production, and fostering a more sustainable form of agriculture.
NAL Thesaurus:	Gene expression Biopesticides
Palavras-chave:	DsRNA RNAi PTGS TGS HIGS SIGS
Digital Object Identifier:	https://doi.org/10.3390/agronomy15040859
Notas:	Na publicação: Maria Fatima Grossi-de-Sa.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CENARGEN)