Title:	Production and characterization of a novel glycolipid biosurfactant from Bradyrhizobium sp.
Authors:	DIAS, M. A. M. ROSSINI, E. L. BRITTO, D. de NITSCHKE, M.
Affiliation:	MARCOS ANDRÉ MOURA DIAS, UNIVERSITY OF SÃO PAULO; EDUARDO LUIZ ROSSINI, UNIVERSITY OF SÃO PAULO; DOUGLAS DE BRITTO, CPATSA; MARCIA NITSCHKE, UNIVERSITY OF SÃO PAULO.
Date Issued:	2024
Citation:	Fermentation, v. 11, n. 8, 471, 2025.
Description:	Biosurfactants (BS) are surface-active compounds synthesized by microorganisms with broad industrial applications. Although BS-producing strains are widely reported, little is known about their production by diazotrophic bacteria. This study investigated, for the first time, the BS produced by Bradyrhizobium sp. ESA 81, a diazotrophic bacterium isolated from the Brazilian semiarid region. The strain was cultivated in the mineral medium using sunflower oil and ammonium nitrate as carbon and nitrogen sources. The compound was chemically characterized using TLC, FAME, FTIR, and mass spectrometry (MALDI-TOF). The results revealed a mixture of glycolipids composed of trehalose linked to fatty acid chains ranging from C9 to C18. The BS exhibited a surface tension of 31.8 mN/m, a critical micelle concentration of 61.2 mg/L, and an interfacial tension of 22.1 mN/m. The BS also showed an emulsification index (EI24) of 55.0%. High stability was observed under extreme conditions of temperature (−20 to 121 °C), pH (2–12), NaCl (5–20%), and sucrose (1–5%). These findings indicate that the trehalolipid BS produced by Bradyrhizobium sp. ESA 81 is a stable and efficient surface-active agent, with promising potential for use in biotechnological and industrial processes.
Thesagro:	Bactéria
NAL Thesaurus:	Bradyrhizobium Biosurfactants
Keywords:	Biossurfactante Trehalolipídio Rizóbio Bactéria diazotrófica Óleo de girassol Biotensoativos BS Bioinseticida
DOI:	https://doi.org/10.3390/fermentation11080471
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)