Title:	Nanotoxicological assessment of green-synthesized silver nanoparticles from Brazilian Cerrado plant in a murine model.
Authors:	BONATTO, C. C. REIS, I. G. RIBEIRO, D. J. S. ALMEIDA, R. das N. CORRÊA, R. DOURADO, L. P. S. PASQUARELLI-DO-NASCIMENTO, G. MAGALHÃES, K. G. SILVA, L. P. da
Affiliation:	CÍNTHIA CAETANO BONATTO, UNIVERSITY OF BRASILIA (UNB) IVY GARCEZ REIS, UNIVERSITY OF BRASILIA (UNB) DALILA JULIANA SILVA RIBEIRO, UNIVERSITY OF BRASILIA (UNB) RAQUEL DAS NEVES ALMEIDA, UMASS CHAN MEDICAL SCHOOL RAFAEL CORRÊA, UNIVERSITY OF BRASILIA (UNB) LIVIA PIMENTEL SANT’ANA DOURADO, UNIVERSITY OF BRASILIA (UNB) GABRIEL PASQUARELLI-DO-NASCIMENTO, UNIVERSITY OF BRASILIA (UNB) KELLY GRACE MAGALHÃES, UNIVERSITY OF BRASILIA (UNB) LUCIANO PAULINO DA SILVA, CENARGEN.
Date Issued:	2025
Citation:	Pharmaceuticals, v. 18, n. 7, 993, 2025.
Description:	In recent years, silver nanoparticles (AgNPs) have garnered significant attention due to their potent antimicrobial properties, which hold promise for various applications. However, concerns about their potential toxicity have also emerged, particularly regarding their impact on human and animal health. This study investigates the acute toxicological effects of AgNPs synthesized using a green route with an aqueous extract of a native Cerrado plant (AgNPs-Cb) in mice. Methods: The AgNPs-Cb were intravenously administered at a concentration of 64 µM, and the mice were euthanized after 24 h for the collection of blood and organ samples (liver, spleen, kidneys, and lungs) for hematological, biochemical, and histological analyses. Results: Hematological analysis, including complete blood count (CBC) and differential leukocyte count, showed no statistically significant alterations in the groups treated with AgNPs-Cb, Cb extract, and Ag+, compared with the control group (p < 0.05). Notably, only the Ag+ group exhibited a significant increase in red blood cell count and hematocrit levels, suggesting that the nanoformulation of silver might mitigate the hematological impact seen with free silver ions. Biochemical analyses of liver and kidney function markers also revealed no significant differences across the treatment groups. Conclusions: These findings indicate that AgNPs-Cb may offer a safer alternative for antimicrobial applications, reducing the risk of acute toxicity in mammals while maintaining efficacy against pathogens. Further studies are needed to explore the underlying mechanisms and long-term effects of AgNPs-Cb exposure.
Thesagro:	Caryocar Brasiliense Cerrado
Keywords:	AgNPs Nanosafety Green synthesis Hematological analysis Biochemical analysis Histological analysis
DOI:	https://doi.org/10.3390/ph18070993
Notes:	Na publicação: Luciano Paulino Silva.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CENARGEN)