Title:	Protective effects of Eugenia uniflora red fruit on brain in a rat model of type 2 diabetes: mechanistic insights.
Authors:	AHMAD, I. CARDOSO, J. de S. MELLO, J. E. de TEIXEIRA, F. C. SARAIVA, J. T. BONA, N. P. VIZZOTTO, M. SOUZA, L. P. de DOMINGUES, W. B. CAMPOS, V. F. LENCINA, C. L. SPANEVELLO, R. M. TAVARES, E. G. AGUIAR, M. S. S. de STEFANELLO, F. M.
Affiliation:	IMTIAZ AHMAD, UNIVERSIDADE FEDERAL DE PELOTAS JULIANE DE SOUZA CARDOSO, UNIVERSIDADE FEDERAL DE PELOTAS JULIA EISENHARDT DE MELLO, UNIVERSIDADE FEDERAL DE PELOTAS FERNANDA CARDOSO TEIXEIRA, UNIVERSIDADE FEDERAL DE PELOTAS JULIANE TORCHELSEN SARAIVA, UNIVERSIDADE FEDERAL DE PELOTAS NATÁLIA PONTES BONA, UNIVERSIDADE FEDERAL DE PELOTAS MARCIA VIZZOTTO FOSTER, CPACT LUCAS PETITEMBERTE DE SOUZA, UNIVERSIDADE FEDERAL DE PELOTAS WILLIAM BORGES DOMINGUES, UNIVERSIDADE FEDERAL DE PELOTAS VINICIUS FARIAS CAMPOS, UNIVERSIDADE FEDERAL DE PELOTAS CLAITON LEONETI LENCINA, UNIVERSIDADE FEDERAL DE PELOTAS ROSELIA MARIA SPANEVELLO, UNIVERSIDADE FEDERAL DE PELOTAS REJANE GIACOMELLI TAVARES, UNIVERSIDADE LUSÓFONA DE HUMANIDADES E TECNOLOGIAS MAYARA SANDRIELLY SOARES DE AGUIAR, UNIVERSIDADE FEDERAL DE PELOTAS FRANCIELI MORO STEFANELLO, UNIVERSIDADE FEDERAL DE PELOTAS.
Date Issued:	2025
Citation:	Neurochemical Research, v. 50, article number 335, 2025.
Description:	The current study aimed to evaluate the in-vitro toxicity and in-vivo antidiabetic effects of a standardized extract from Eugenia uniflora (E. uniflora) fruit, comparing its efficacy to metformin (Met), a widely used anti-hyperglycemic drug. First, toxicity of the extract was determined by MTT assay in 3T3 cell line and primary astrocyte culture. Then Wistar rats were divided into four groups: I- Control, II- type 2 diabetes mellitus (T2DM), III- T2DM + Met and IV- T2DM + E. uniflora. To induce T2DM, groups II, III and IV received a high fat diet (HFD) for 3 weeks followed by a single intraperitoneal (i.p.) dose of streptozotocin (STZ, 35 mg/kg). Animals of group I received normal diet and vehicle (i.p.). Group III received Met (250 mg/kg) and group IV received E. uniflora (200 mg/kg) intragastric pathway, once a day, throughout all experimental protocol. Animals from the groups I and II received water in the same volume. Results showed that cell viability was not affected. In-vivo, E. uniflora and Met prevented the change in serum levels of glucose, cholesterol, LDL, triglyceride and interleukin-6. Furthermore, extract and Met improved oxidative stress markers and antioxidant enzyme activity in the brain (cerebral cortex, hippocampus, striatum). Furthermore, extract enhanced the downstream insulin signaling pathway, including insulin receptor substrate 1, forkhead box protein O-3a, as well as activated the nuclear factor erythroid 2-related factor 2 in the cerebral cortex. This study indicates that E. uniflora extract may have potential in preventing complications associated with T2DM; nevertheless, additional studies are required to confirm these effects and establish their clinical significance.
Thesagro:	Medicamento Homeopático Eugenia Uniflora Insulina Cérebro
NAL Thesaurus:	Hyperglycemia Insulin Interleukin-6 Brain
Keywords:	Interleucina-6 Diabetes tipo 2 Hiperglicemia Type 2 diabetes
DOI:	https://doi.org/10.1007/s11064-025-04587-5
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPACT)