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dc.contributor.authorVIEIRA, C. D.
dc.contributor.authorLEMOS, D. R. de
dc.contributor.authorPEREIRA, H. P.
dc.contributor.authorSOUZA, E. D. de
dc.contributor.authorSARAIVA, N. Z.
dc.contributor.authorSIQUEIRA, L. G. B.
dc.contributor.authorOLIVEIRA, C. S.
dc.contributor.authorQUINTAO, C. C. R.
dc.contributor.authorCAMARGO, L. S. de A.
dc.date.accessioned2025-11-24T19:52:40Z-
dc.date.available2025-11-24T19:52:40Z-
dc.date.created2025-11-24
dc.date.issued2025
dc.identifier.citationIn: INTERNATIONAL CONGRESS OF THE BRAZILIAN GENETICS SOCIETY, 70., 2025, Belém. E-book: [abstracts]. Ribeirão Preto: Sociedade Brasileira de Genética, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181829-
dc.descriptionGene editing offers opportunities for the genetic improvement of animals. However, mosaicism continues to represent a significant challenge in genetic modification experiments, due to the presence of two or more cell lines with distinct genotypes within the same organism, hindering both accurate genotyping and reliable transmission of mutations to offspring. In this context, preimplantation genetic screening of embryos emerges as a promising strategy. In the present study, the CRISPR/Cas9 system combined with electroporation (EP) using a Nepa21 electroporator (Nepagene, Japan) was employed to edit the beta-lactoglobulin (BLG) gene in in vitro-fertilized bovine oocytes. EP of the sgRNA and Cas9 components was carried out 8 hours after fertilization
dc.language.isoeng
dc.rightsopenAccess
dc.subjectEdição genética
dc.titleEmbryo biopsy as a tool for preimplantation identification of beta-lactoglobulin-edited bovine embryos.
dc.typeResumo em anais e proceedings
dc.subject.thesagroBovino
dc.subject.thesagroEmbrião Animal
dc.subject.thesagroMelhoramento Genético Animal
dc.subject.thesagroBiopsia
dc.format.extent2p. 236.
riaa.ainfo.id1181829
riaa.ainfo.lastupdate2025-11-24
dc.contributor.institutionCAROLINA DAVID VIEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DIANA RANGEL DE LEMOS, UNIVERSIDADE FEDERAL DE VIÇOSA; HYAGO PASSE PEREIRA; ELIZA DINIZ DE SOUZA; NAIARA ZOCCAL SARAIVA, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Aparece en las colecciones:Resumo em anais de congresso (CNPGL)

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