Title:	Embryo biopsy as a tool for preimplantation identification of beta-lactoglobulin-edited bovine embryos.
Authors:	VIEIRA, C. D. LEMOS, D. R. de PEREIRA, H. P. SOUZA, E. D. de SARAIVA, N. Z. SIQUEIRA, L. G. B. OLIVEIRA, C. S. QUINTAO, C. C. R. CAMARGO, L. S. de A.
Affiliation:	CAROLINA DAVID VIEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DIANA RANGEL DE LEMOS, UNIVERSIDADE FEDERAL DE VIÇOSA; HYAGO PASSE PEREIRA; ELIZA DINIZ DE SOUZA; NAIARA ZOCCAL SARAIVA, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Date Issued:	2025
Citation:	In: INTERNATIONAL CONGRESS OF THE BRAZILIAN GENETICS SOCIETY, 70., 2025, Belém. E-book: [abstracts]. Ribeirão Preto: Sociedade Brasileira de Genética, 2025.
Pages:	p. 236.
Description:	Gene editing offers opportunities for the genetic improvement of animals. However, mosaicism continues to represent a significant challenge in genetic modification experiments, due to the presence of two or more cell lines with distinct genotypes within the same organism, hindering both accurate genotyping and reliable transmission of mutations to offspring. In this context, preimplantation genetic screening of embryos emerges as a promising strategy. In the present study, the CRISPR/Cas9 system combined with electroporation (EP) using a Nepa21 electroporator (Nepagene, Japan) was employed to edit the beta-lactoglobulin (BLG) gene in in vitro-fertilized bovine oocytes. EP of the sgRNA and Cas9 components was carried out 8 hours after fertilization
Thesagro:	Bovino Embrião Animal Melhoramento Genético Animal Biopsia
Keywords:	Edição genética
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPGL)