Título:	Assessing the immune response of different ß-globin haplotypes and breed in Haemonchus contortus infected sheep.
Autor:	CANALLI, G. F. D. ALENCAR, M. F. D. de BELLO, H. J. S. OKINO, C. H. COSTA, E. C. da CUNHA, A. F. da KAPRITCHKOFF, R. T. I. CORRÊA, L. G. P. CHAGAS, A. C. de S. ALBUQUERQUE, A. C. A. de
Afiliación:	GUILHERME FERNANDES DIAS CANALLI, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; MARIA FLÁVIA DIAS DE ALENCAR, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; HORNBLENDA JOAQUINA SILVA BELLO, FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO; CINTIA HIROMI OKINO, CPPSE; ESTEVÃO CAMILLO DA COSTA, CENTRO UNIVERSITÁRIO CENTRAL PAULISTA; AMANDA FREITAS DA CUNHA, CENTRO UNIVERSITÁRIO CENTRAL PAULISTA; RAFAELA TAMI IKEDA KAPRITCHKOFF, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; LORRANE GABRIELE PINHEIRO CORRÊA, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; ANA CLÁUDIA ALEXANDRE DE ALBUQUERQUE, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO.
Año:	2025
Referencia:	In: CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENTS OF VETERINARY PARASITOLOGY, 30., 2025, Curitiba. Abstract book. Curitiba: Universidade Federal do Paraná, 2025. p. 251-252.
Descripción:	Abstract: Sheep exhibit two distinct β-globin haplotypes, Hb-A and Hb-B. During anemic conditions, Hb-A animals can express juvenile βC, which displays a higher affinity for oxygen, an ability absent in Hb-B animals. β-globin haplotypes have been associated with variable sheep resistance/resilience to gastrointestinal nematode infection. This research aimed to compare the adaptive immune response in the abomasum of lambs of different breeds and β-globin haplotypes artificially infected by Haemonchus contortus. Twenty-one male lambs, comprising 12 Santa Inês (SI), 5 Texel (TX), and 4 White Dorper (DO), with six months old, were selected from the Embrapa Southeastern Livestock experimental farm based on their β-globin types (AA, AB, BB) through blood DNA analysis. Subsequently, those lambs were dewormed and received a single dosage of 4,000 H. contortus L3. Twenty-eight days post-infection, they were slaughtered, and the fundic region of abomasum tissue was formalin-fixed and paraffinembedded for immunohistochemistry analysis of MHC II (HLA-DR) and T lymphocytes (CD3) cells. Positive cell counting was performed in ten random fields under a 40x objective with a 1 mm² graticule. There was no effect of haplotype in MHC II+ (p = 0.980) (AA: 248±60.6; AB: 234±47.1 and BB: 254±121.8) and in CD3+ means (p = 0.140) (AA: 1122±304.9; AB: 976±820.3 and BB: 520±595.5). Similar results were observed for breed effect in MHC II+ (p = 0.130) (DO: 162±77.7; SI: 263±83.3 and TX: 280.6±114.5) and CD3+ means (p = 0.787) (DO: 1360±278.9; SI: 1233±778.2 and TX: 1473±497.4). The limited presence of AA animals in the flock may have influenced the absence of differences among haplotypes. This similar immunological response is being better investigated with a larger number of animals, as there seemed to be a tendency for AA animals to have a higher average of CD3+, along with a breed effect in MHC II+ of SI and TX compared to DO.
Thesagro:	Ovelha Haemonchus Contortus
NAL Thesaurus:	Haplotypes Lambs Abomasum Haemonchus Immune response
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CPPSE)