Title:	Development of a β-glucosidase improved for glucose retroinhibition for cellulosic ethanol production: an integrated bioinformatics and genetic engineering approach.
Authors:	AZEVEDO, R. dos S. SANTANA, H. SEUS, V. R. CARMAGO, A. D. WERHLI, A. V. MACHADO, K. dos S. JUNGMANN, L. QUIRINO, B. F. MARINS, L. F.
Affiliation:	RAÍZA DOS SANTOS AZEVEDO, FEDERAL UNIVERSITY OF RIO GRANDE; HUGO SANTANA, UNIVERSITY OF BRASÍLIA; VINÍCIUS ROSA SEUS, FEDERAL UNIVERSITY OF RIO GRANDE; ALEX DIAS CAMARGO, FEDERAL UNIVERSITY OF RIO GRANDE; ADRIANO VELASQUE WERHLI, FEDERAL UNIVERSITY OF RIO GRANDE; KARINA DOS SANTOS MACHADO, FEDERAL UNIVERSITY OF RIO GRANDE; LETICIA JUNGMANN CANCADO, CNPAE; BETANIA FERRAZ QUIRINO, CNPAE; LUIS FERNANDO MARINS, FEDERAL UNIVERSITY OF RIO GRANDE.
Date Issued:	2025
Citation:	Biotechnology for Biofuels and Bioproducts, v. 18, 2025.
Description:	In this work, a variant of a β-glucosidase from the GH1 family, isolated from the microbial community of Amazonian soil (Brazil), with enhanced catalytic activity and improved for glucose retroinhibition, was developed. This new variant may become a promising alternative to reduce the costs of enzyme cocktails used in the hydrolysis of lignocellulosic biomass used as a raw material in the production of second-generation ethanol.
NAL Thesaurus:	Cellulases Catalytic activity Enzymatic hydrolysis Cellulose
Keywords:	Genetic manipulation Lignocellulosic biomass
DOI:	https://doi.org/10.1186/s13068-025-02643-4
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAE)