Title:	Standardization of sporulation and inoculation methodologies and reaction of acerola genotypes to dieback.
Authors:	BRANDÃO, L. A. HONORATO, A. da C. SILVA, A. H. da CABFRAL, P. G. C. SOUZA, F. de F. CAPUCHO, A. S.
Affiliation:	LEONARDO APARECIDO BRANDÃO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; ALAN DA CUNHA HONORATO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; ANTONIO HELTON DA SILVA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; PATRÍCIA GONÇALVES CASTRO CABRAL, INSTITUTO FEDERAL SERTÃO PERNAMBUCANO; FLAVIO DE FRANCA SOUZA, CPATSA; ALEXANDRE SANDRI CAPUCHO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO.
Date Issued:	2025
Citation:	Arquivos do Instituto Biológico, v. 92, e0062024, 2025.
Description:	Acerola cultivation has been expanding in Brazilian Northeast region. However, diseases caused by Botryosphaeriaceae fungi, such as dieback, may affect acerola orchards. This study aimed to standardize sporulation and inoculation methods, to assess Lasiodiplodia sp. aggressiveness in three hosts, and resistance of acerola genotypes to dieback. Five substrates obtained from host species and five Botryosphaeriaceae isolates were used to induce sporulation, and three inoculation methods were assessed. The aggressiveness of eight Lasiodiplodia strains isolated from acerola was compared in mango, acerola, and grapevine seedlings and fruits. The resistance of 34 acerola genotypes was assessed after inoculation with an aggressive Lasiodiplodia strain. Sporulation was higher in the mango leaf and acerola branch, and the cork-borer method was the easiest and fastest one. Acerola was the most susceptible host, and there was a difference in the aggressiveness among Lasiodiplodia sp. strains. There was a difference in susceptibility to dieback among acerola genotypes. This study provides alternative methodologies for sporulation and inoculation of Botryosphaeriaceae fungi and resistant acerola genotypes to dieback for further use in breeding programs.
Thesagro:	Acerola Melhoramento Genético Vegetal Resistência Genética Fungo
NAL Thesaurus:	Botryosphaeriaceae Plant pathology Genetic resistance
Keywords:	Cereja-das-antilhas Cereja de barbados
DOI:	https://doi.org/10.1590/1808-165700062024
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)