Title:	Structural and viability assessment of bovine corneas preserved at an accessible low-temperature (−20 °C) for eye irritation models.
Authors:	ONORATO, G. REIS JUNIOR, J. L. BRANDAO, H. de M. MUNK, M.
Affiliation:	GEOVANA ONORATO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JANILDO LUDOLF REIS JUNIOR, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, UNIVERSIDADE FEDERAL DE JUIZ DE FORA.
Date Issued:	2025
Citation:	ACS Omega, v. 10, p. 46759-46768, 2025.
Description:	In vitro ocular irritation testing requires the use of freshly excised bovine corneas, which limits broader adoption in laboratories with restricted access to slaughterhouse material. This study investigated a practical and accessible lowtemperature preservation strategy focused on maintaining structural and cellular integrity. Bovine corneas were stored at −20 or −80 °C using two commonly available cryoprotective agents: dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Preservation outcomes were assessed through corneal transparency, histological architecture, and endothelial viability. Among the tested conditions, storage at −20 °C with 10% DMSO provided the most consistent preservation of structural morphology and transparency. This protocol provides a cost-effective foundation for extending the usability of excised corneas and supports future efforts to expand the accessibility of in vitro eye irritation models in laboratories with limited infrastructure.
Thesagro:	Bovino
NAL Thesaurus:	Cornea
Keywords:	Irritação ocular
DOI:	https://doi.org/10.1021/acsomega.5c04314
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)