Título:	Production of Polymeric Membranes Functionalized with Vitreous Humor and Secretome from Dental Pulp Stem Cells for Use in Epithelial Regeneration.
Autoria:	BARROSO, W. da S. LUZ, E. P. C. G. MARQUES, L. S. M. CAVALCANTE, P. E. da S. SOUZA, F. F. P. de MATTOS, A. L. A. ANDRADE, F. K. SILVA, A. L. C. da HIRATA, M. C. BOSCO, I. ROCHA, D. N. da SOUZA, R. F. B. de SOUZA, F. C. B. de FERREIRA, J. R. M. VIEIRA, R. S.
Afiliação:	WALLADY DA SILVA BARROSO, FEDERAL UNIVERSITY OF CEARÁ ERIKA PATRÍCIA CHAGAS GOMES LUZ, FEDERAL UNIVERSITY OF CEARÁ LIDYANE SOUTO MACIEL MARQUES, FEDERAL UNIVERSITY OF CEARÁ PAULO EDUARDO DA SILVA CAVALCANTE, FEDERAL UNIVERSITY OF CEARÁ FRANCISCO FÁBIO PEREIRA DE SOUZA, FEDERAL UNIVERSITY OF CEARÁ ADRIANO LINCOLN ALBUQUERQUE MATTOS, CNPAT FABIA KARINE ANDRADE, FEDERAL UNIVERSITY OF CEARÁ ANDRÉ LUÍS COELHO DA SILVA, FEDERAL UNIVERSITY OF CEARÁ MARIÁH CATIONI HIRATA, R-CRIO STEM CELLS ISADORA BOSCO, R-CRIO STEM CELLS DANIEL NAVARRO DA ROCHA, R-CRIO STEM CELLS RENATA FRANCIELLE BOMBALDI DE SOUZA, R-CRIO STEM CELLS FERNANDA CARLA BOMBALDI DE SOUZA, R-CRIO STEM CELLS JOSÉ RICARDO MUNIZ FERREIRA, R-CRIO STEM CELLS RODRIGO SILVEIRA VIEIRA, FEDERAL UNIVERSITY OF CEARÁ.
Ano de publicação:	2025
Referência:	ACS Applied Bio Materials, 8, 7011−7022, 2025.
Conteúdo:	This study investigated the development of porous membranes based on a matrix of bacterial cellulose (BC) and sodium alginate (ALG) incorporated with proteins extracted from the tuna vitreous humor and complete conditioned medium (CCM) obtained from the in vitro culture of dental pulp stem cells. These bioactive components were integrated into BC-ALG membranes, which were then evaluated for morphology, physicochemical properties, in vitro cytotoxicity, and regenerative potential in vitro. The membranes exhibited high porosity (72% to 89%), and remarkable swelling capacity reaching up to 3376 ± 0.78% in 72 min, indicating excellent moisture retention essential for wound healing in vivo. Cytotoxicity assay showed that none of the formulations were cytotoxic to the tested mammalian cells, confirming their cytocompatibility. Membranes containing CCM showed enhanced cell viability at lower concentrations (500 μg/ mL and 250 μg/mL). In vitro scratch assays demonstrated that membranes with vitreous humor (VH) and CCM reduced scratch closure time from 18 to 12 h, suggesting their potential for accelerating skin regeneration. This work underscores the promise of using sustainable, waste-derived materials for creating bioactive membranes with applications in tissue engineering and regenerative medicine
Palavras-chave:	Bacterial cellulose Vitreous humor Biological waste Wound dressings Secretome
Digital Object Identifier:	https://doi.org/10.1021/acsabm.5c00743
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAT)