Título:	Evaluation of nanoatrazine bioaccumulation in green microalga Raphidocelis subcapitata: characterization, stability and uptake analysis.
Autor:	BRAGA, A. P. A. VALLIM, J. H. MENDES, M. D. L. LEVANTEZA, V. O. ASSALIN, M. R. TAVARES, M. M. PEREIRA, A. E. S. FRACETO, L. F. CASTRO, V. L. S. S. de
Afiliación:	A. P. A. BRAGA; JOSE HENRIQUE VALLIM, CNPMA; MARA DENISE LUCK MENDES, CNPMA; V. O. LEVANTEZA; MARCIA REGINA ASSALIN, CNPMA; MARLEY MENDONCA TAVARES, CNPMA; A. E. S. PEREIRA; L. F. FRACETO; VERA LUCIA SCHERHOLZ SALGADO DE CASTRO, CNPMA.
Año:	2025
Referencia:	Toxicology Letters, v. 411, Supplement, p. S110, 2025. Abstracts of the 59th Congress of the European Societies of Toxicology (EUROTOX), Athens, Greece, 2025.
Páginas:	1 p.
Descripción:	The use of nanoencapsulated pesticides, such as nanoatrazine (nATZ), has been proposed as a promising strategy to enhance herbicidal efficiency and reduce environmental toxicity. However, concerns remain regarding their environmental fate and bioaccumulation potential in non-target organisms, especially within aquatic food webs. This study aims to investigate the behavior and initial bioaccumulation of nATZ encapsulated in poly(E- caprolactone) (PCL) nanoparticles in the freshwater microalga Raphidocelis subcapitata, as a primary step toward understanding its trophic transfer. The nATZ-PCL nanoparticles were synthesized via nanoprecipitation and characterized using dynamic light scattering (DLS). The resulting particles exhibited an average size of 226 nm, a polydispersity index (PDI) of 0.12, and a zeta potential of -25 mV, indicating a stable and monodisperse suspension. Additionally, stability assessments in different exposure media confirmed that the physicochemical properties of the nanoparticles were maintained under experimental conditions. In the bioaccumulation assays, R. subcapitata cultures were exposed for 7 days to a defined concentration of 0.103 mg/L of nATZ-PCL, corresponding to the previously determined effective concentration (EC25). Quantification of intracellular atrazine was performed via high-performance liquid chromatography (HPLC), revealing a concentration of 1.68 μg/g dry weight, confirming cellular uptake of the nanoencapsulated herbicide. These preliminary findings suggest that nATZPCL is bioavailable to primary producers and can potentially enter the aquatic trophic chain. These results highlight the importance of monitoring the fate and effects of nanoformulations in aquatic systems. Further experiments involving Daphnia magna and Danio rerio are currently underway to assess trophic transfer and potential sublethal effects. Biochemical responses, including antioxidant enzyme activities, are being proceeded to evaluate biomarkers effects. Additionally, exposure scenarios will include the presence of natural organic matter (NOM) to better simulate environmentally relevant conditions. This research is expected to contribute to the establishment of more accurate environmental risk assessment strategies for nanopesticides, supporting regulatory advances and sustainable practices in agriculture.
Thesagro:	Microalga
NAL Thesaurus:	Bioaccumulation Bioaccumulation factor
Palabras clave:	Raphidocelis subcapitata Nano atrazina
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CNPMA)