Título:	Genetic diversity and structure in Coffea canephora genotypes from the Amazon Region.
Autoria:	FERNANDES, S. H. F. BEZERRA, C. de S. FERREYRA RAMOS, S. L. LOPES, R. ESPINDULA, M. C. RAMOS, T. S. MATIELLO, R. R. MARQUES, M. J. MENESES, C. H. S. G. LOPES, M. T. G.
Afiliação:	SHAYENNE HEVELYN FARIAS FERNANDES, UNIVERSIDADE FEDERAL DO AMAZONAS; CAROLINE DE SOUZA BEZERRA, UNIVERSIDADE FEDERAL DO AMAZONAS; SANTIAGO LINORIO FERREYRA RAMOS, UNIVERSIDADE FEDERAL DO AMAZONAS; RICARDO LOPES, CPAA; MARCELO CURITIBA ESPINDULA, CPAFRO; THAYNARA SILVA RAMOS, UNIVERSIDADE ESTADUAL DA PARAÍBA; RODRIGO RODRIGUES MATIELLO, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; MARIA JOSÉ MARQUES, UNIVERSIDADE FEDERAL DO AMAZONAS; CARLOS HENRIQUE SALVINO GADELHA MENESES, UNIVERSIDADE ESTADUAL DA PARAÍBA; MARIA TERESA GOMES LOPES, UNIVERSIDADE FEDERAL DO AMAZONAS.
Ano de publicação:	2026
Referência:	Horticulturae, v. 12, n. 2, art. 250, 2026.
Conteúdo:	Coffea canephora is economically and socially important for small-scale agriculture in Northern Brazil. To identify genotypes adapted to Amazonian edaphoclimatic conditions, clones of the species have been evaluated across multiple locations in Amazonas. Introducing genetically selected materials into comparable environments may promote consistent productivity gains in the short and medium term. In this context, the aim of this study was to assess the genetic diversity of different C. canephora genotypes using microsatellite markers, which will support the development of superior genotypes adapted to Amazon conditions. A total of 43 C. canephora genotypes were analyzed. Leaves were collected for genomic DNA extraction and were standardized and amplified by PCR using microsatellite primers. Genotyping was performed via capillary electrophoresis, allowing for the determination of allele sizes. Genetic structure was inferred, and genetic diversity parameters were estimated. The average observed heterozygosity (HO = 0.64) exceeded the expected heterozygosity (HE = 0.53), and the average inbreeding coefficient (f = −0.19) indicated an excess of heterozygotes. The results revealed high genetic variability among the evaluated genotypes. These findings highlight the broad genetic diversity of C. canephora, reinforcing its potential as a genetic basis for selection and the development of cultivars adapted to the environmental conditions of the Amazon.
Thesagro:	Coffea Canephora Marcador Molecular Banco de Germoplasma Café
NAL Thesaurus:	Heterozygosity
Palavras-chave:	Molecular markers Germplasm bank Genetic structure Diversidade genética Variabilidade genética
Digital Object Identifier:	https://doi.org/10.3390/horticulturae12020250
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAA)