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dc.contributor.authorSABEDOTTI, C.
dc.contributor.authorSILVEIRA, I. DA
dc.contributor.authorMIGACZ, I. P.
dc.contributor.authorCRUZ, L. S.
dc.contributor.authorVENTURA, A. C. T.
dc.contributor.authorFARAGO, P. V.
dc.contributor.authorMONDIN, C. A.
dc.contributor.authorRAMAN, V.
dc.contributor.authorBRITO, E. S. de
dc.contributor.authorRIBEIRO, P. R. V.
dc.contributor.authorBELTRAME, F. L.
dc.contributor.authorMANFRON, J.
dc.date.accessioned2026-05-05T12:48:33Z-
dc.date.available2026-05-05T12:48:33Z-
dc.date.created2026-05-05
dc.date.issued2026
dc.identifier.citationRevista Brasileira de Farmacognosia, v. 36, n. 47, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1186595-
dc.descriptionBidens pilosa L., Bidens alba (L.) DC., and Bidens subalternans DC., Asteraceae, are often misidentified because of their morphological similarities. The present study provides a comparative analysis of the morpho-anatomical characteristics, histochemical, and chemical fingerprints obtained by liquid chromatography mass spectrometry for these three species. Light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy were used to characterize diagnostic structures. Histochemical assays and LC-MS were applied to obtain chemical information from leaf, stem, and root extracts, and multivariate chemometrics including principal component analysis were used to classify the datasets. The morpho-anatomical and histochemical analysis allowed the identification and differentiation of the three species; also, the LC-MS analysis revealed that the main compounds in Bidens extracts are phenolics, and using chemometrics methods was possible to discriminate by species and plant parts. Together, these complementary approaches provide a robust basis for distinguishing closely related Bidens species and support more reliable pharmacognostic identification.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectHerbals
dc.subjectMicromorphology
dc.subjectPharmacobotany
dc.subjectSpecies authentication
dc.titleExploring anatomical and chemical markers to characterize and differentiate among Bidens species.
dc.typeArtigo de periódico
dc.subject.nalthesaurusChemotaxonomy
dc.subject.nalthesaurusQuality control
riaa.ainfo.id1186595
riaa.ainfo.lastupdate2026-05-05
dc.identifier.doi10.1007/s43450-026-00759-8
dc.contributor.institutionCAROLINA SABEDOTTI, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; ISABELLA DA SILVEIRA, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; IZABEL PIETCZAK MIGACZ, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; LUIZA STOLZ CRUZ, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE; ANA CAROLINA TERSO VENTURA, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; PAULO VITOR FARAGO, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; CLAUDIO AUGUSTO MONDIN, INSTITUTO CONECTAR AMBIENTAL; VIJAYASANKAR RAMAN, DEPARTMENT OF AGRICULTURE, DISTRICT OF COLUMBIA, NATIONAL IDENTIFICATION SERVICES, WASHINGTON, USA; EDY SOUSA DE BRITO, CNAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; FLÁVIO LUÍS BELTRAME, UNIVERSIDADE ESTADUAL DE PONTA GROSSA; JANE MANFRON, UNIVERSIDADE ESTADUAL DE PONTA GROSSA.
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